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Regulation of Growth and Biosynthetic Activity of the Medicinal Jelly Mushroom Tremella mesenterica (Retz.: Fr.) Pure Culture

2001; Begell House; Volume: 3; Issue: 1 Linguagem: Inglês

10.1615/intjmedmushr.v3.i1.70

1521-9437

Sergey V. Reshetnikov, Ina Duckman, Katherina Tsukor,

Mycorrhizal Fungi and Plant Interactions

Different yeast-like budding haploid strains of the yellow brain mushroom Tremella mesenterica (Retz.: Fr.) were obtained using the monobasidiosporous culture method from basidioma specimens collected in Israel. Nutritional requirements for biomass growth and extracellular polysaccharide production were investigated. A two-stage technology for acidic glucuronoxylomannan production was developed. On a first stage inoculum culture medium was balanced for biomass accumulation, and maximal polysaccharide production was obtained on a fermentation medium under conditions of nitrogen limitation. A crude product obtained by alcohol precipitation of culture broth contains 40% of acidic glucuronoxylomannan, 5% of neutral glucuronoxylomannan, 30% of cell biomass, and also includes low molecular weight substances, free amino acids, and mineral elements.