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A cell strain cultured from porcine kidney increases cyclic AMP content upon exposure to calcitonin or vasopressin

1978; Elsevier BV; Volume: 83; Issue: 2 Linguagem: Inglês

10.1016/0006-291x(78)91009-4

1090-2104

Steven R. Goldring, Jean‐Michel Dayer, Dennis A. Ausiello, Stephen M. Krane,

Veterinary Medicine and Surgery

Cells originally dispersed from whole juvenile male Hampshire pig kidney and maintained in monolayer culture, increased cyclic AMP content in response to incubation with salmon calcitonin or antidiuretic hormone. Parathyroid hormone and epinephrine did not affect cyclic AMP content. The apparent Km for arginine vasopressin in the porcine cells was 3.0 nM which is similar to the value obtained in single segments of rabbit kidney tubule. The apparent Km for salmon calcitonin of 2.7 nM is higher than that reported for the rabbit nephron segments, but comparable to the Km obtained in rat kidney homogenates. Exposure of the porcine cells to exogenous prostaglandin E2 did not affect cyclic AMP responses to other hormones. In the cultured porcine kidney cells the pattern of hormone response is similar to that observed in nephron segments prepared from the medullary portion of the thick ascending limb of the loop of Henle, and these findings suggest that the porcine cells may be related to cells present in the medullary region of the kidney tubule.