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Molecularly imprinted solid-phase extraction of (−)-ephedrine from Chinese Ephedra

2005; Elsevier BV; Volume: 1070; Issue: 1-2 Linguagem: Inglês

10.1016/j.chroma.2005.03.017

1873-3778

Xiangchao Dong, Wei Wang, Shujuan Ma, Hui Sun, Yan Li, Jingqiang Guo,

Forensic Toxicology and Drug Analysis

Method of molecularly imprinted solid phase extraction (MISPE) of (-)-ephedrine from Chinese Ephedra has been developed in the research. The molecularly imprinted polymer (MIP) with good selectivity and affinity for (-)-ephedrine was synthesized with (-)-ephedrine as the template, methacrylic acid as the functional monomer. The washing and elution conditions in MISPE were selected and optimized for efficient analyte extraction and sample clean-up. A clean analytical HPLC base line of ephedra extract was obtained after MISPE, which indicated that the sample pre-treatment was efficient. Good recovery and precision were obtained in the assessment for the MISPE-HPLC procedure, which demonstrated it is a reliable method and can be used for the determination of (-)-ephedrine in herbal ephedra.