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First Report of Pepper vein yellows virus Infecting Red Pepper in Mainland China

2015; American Phytopathological Society; Volume: 99; Issue: 8 Linguagem: Inglês

10.1094/pdis-01-15-0025-pdn

1943-7692

S. B. Zhang, Zijin Zhao, D. Y. Zhang, Y. Liu, S. B. Zhang, D. Y. Zhang, Y. Liu, Xin-Chao Luo, J. Liu, Like Wu, Jing Peng,

Plant and Fungal Interactions Research

HomePlant DiseaseVol. 99, No. 8First Report of Pepper vein yellows virus Infecting Red Pepper in Mainland China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Pepper vein yellows virus Infecting Red Pepper in Mainland ChinaS. B. Zhang, Z. B. Zhao, D. Y. Zhang, Y. Liu, S. B. Zhang, D. Y. Zhang, Y. Liu, X. W. Luo, J. Liu, L. F. Wu, and J. PengS. B. Zhang, Z. B. Zhao, D. Y. Zhang, Y. Liu, S. B. Zhang, D. Y. Zhang, Y. Liu, X. W. Luo, J. Liu, L. F. Wu, and J. PengAffiliationsAuthors and Affiliations S. B. Zhang Z. B. Zhao D. Y. Zhang Y. Liu , Shaoyang University, Shaoyang 422000, China S. B. Zhang D. Y. Zhang Y. Liu X. W. Luo J. Liu L. F. Wu J. Peng , Key Laboratory of Pest Management of Horticultural Crop of Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural Science, Changsha 410125, China. Published Online:23 Jun 2015https://doi.org/10.1094/PDIS-01-15-0025-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Pepper vein yellows virus (PeVYV), a novel Polerovirus in the Luteoviridae has been reported in Europe (including Spain and the Netherlands) (Villanueva et al. 2013; Rast 1988), Asia (India, Indonesia, Philippines, Thailand, Japan, and Taiwan), and Africa (Mali) (Knierim et al. 2013; Yonaha et al. 1995). In June 2013, six samples of red pepper (Capsicum frutescens L.) were sampled in three greenhouses in Changsha city, Hunan Province, China, for viral-like symptoms. Of these, three of the sampled red pepper plants displayed symptoms in common, which were similar to those that infected by PeVYV, also designated as Pepper yellow leaf curl virus (PYLCV) and Pepper yellows virus (PYV) at one time (Dombrovsky et al. 2010). Symptoms included dwarfing of the whole plant, leaf curling upward and inward, interveinal yellows, and abnormal shape along the midrib of leaves. Electron microscopy of negative stained crude leaf extracts from the three samples showed sparse yet typical icosahedral virions (28 nm in diameter, consistent with the size of Polerovirus virions). The three red pepper plants were tested for the virus by high-throughput small RNA sequencing. Briefly, two leaves for each red pepper plant were collected and pooled. Total RNA was extracted by Trizol reagent (Sangon Biotech, Shanghai, China). Small RNA libraries were constructed using the Small RNA v1.5 Sample Prep kit (Illumina, San Diego, California, USA) and sequenced using high sequencing by Solexa sequencer (Illumina). The raw data were analyzed using the Illumina GA Pipeline v1.3. High-quality small RNA reads (18 to 30 nt) were assembled to contigs using Velvet 1.0.5 software. The contigs were identified by NBLAST against the database of viral genomes in GenBank (http://www.ncbi.nlm.nih.gov/genomes/GenomesHome.cgi?). There were 1 to 50 contigs highly identical (>90%) to partial sequences of reference genomes of 13 viruses, including PeVYV, in the viral genomes database. To validate the small RNA sequencing data using a different approach, a coat protein (CP) fragment of PeVYV was amplified using the primer pair (PeF: 5′-GGAGCGTTGCGGAATGGATGC-3′, PeR: 5′-TCGCTTGCCCGCCTTTGGTG-3′). Reverse transcription (RT)-PCR was performed from three symptomatic samples and one healthy sample as a negative control. We observed a DNA fragment of excepted size (351 bp) in all three symptomatic samples and not in the healthy sample. The fragment of one randomly selected sample was purified and sequenced. The nucleotide identity of the amplified fragment (GenBank Accession No. KM229707) was 99% with PeVYV isolates from Taiwan (JX427542 and JX427543). Northern blot also showed the existence of PeVYV in one randomly selected sample using a digoxigenin-labeled PCR fragment by primer PeF/PeR against total RNA. The spread of PeVYV in Hunan Province, China is evidence that this virus is spreading from the field where it was first discovered (Yonaha et al. 1995). Our finding supports other findings that this virus threatens pepper and other Solanaceae crops across the globe. It has been reported infecting pepper and nightshade (Solanum nigrum) in several districts and countries in Southeast Asia, Africa, and Europe in 2013 (Villanueva et al. 2013; Knierim et al. 2013).References:Dombrovsky, A., et al. 2010. Phytoparasitica 38:477.https://doi.org/10.1007/s12600-010-0120-x Crossref, ISI, Google ScholarKnierim, D., et al. 2013. Arch. Virol. 158:1337.https://doi.org/10.1007/s00705-012-1598-y Crossref, ISI, Google ScholarRast, A. Th. B. 1988. Neth. J. Plant Pathol. 94:311.https://doi.org/10.1007/BF01998059 Crossref, ISI, Google ScholarVillanueva, F., et al. 2013. Plant Dis. 97:1261.https://doi.org/10.1094/PDIS-04-13-0369-PDN Link, ISI, Google ScholarYonaha, T., et al. 1995. Jpn. J. Phytopathol. 61:178.https://doi.org/10.3186/jjphytopath.61.178 Crossref, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 99, No. 8 August 2015SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 6 Aug 2015Published: 23 Jun 2015First Look: 16 Mar 2015Accepted: 12 Mar 2015 Pages: 1190-1190 Information© 2015 The American Phytopathological SocietyCited byKey Amino Acids for Pepper Vein Yellows Virus P0 Protein Pathogenicity, Gene Silencing, and Subcellular Localization13 September 2021 | Frontiers in Microbiology, Vol. 12First evidence showing that Pepper vein yellows virus P4 protein is a movement protein30 March 2020 | BMC Microbiology, Vol. 20, No. 1First report of Carpesium abrotanoides L. as a natural host plant for pepper vein yellows virus in China31 August 2020 | Journal of Plant Pathology, Vol. 102, No. 4Capsicum annuum and Capsicum frutescens (Bell pepper, Chilli, Pepper, Sweet pepper)6 June 2020Evidence for a complex of emergent poleroviruses affecting pepper worldwide25 January 2018 | Archives of Virology, Vol. 163, No. 5First Report of Pepper vein yellows virus Infecting Tobacco (Nicotiana tabacum) Naturally in ChinaL. 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