Portal de Periódicos da CAPES

iPSC ‐derived human mesenchymal stem cells improve myocardial strain of infarcted myocardium

2014; Wiley; Volume: 18; Issue: 8 Linguagem: Inglês

10.1111/jcmm.12351

1582-4934

Qingfeng Miao, Winston Shim, Nicole Tee, Sze Yun Lim, Ying Ying Chung, K P Myu Mia Ja, Ting Huay Ooi, Grace Tan, Geraldine Kong, Heming Wei, Chong Hee Lim, Yoong Kong Sin, Philip Wong,

Electrospun Nanofibers in Biomedical Applications

Abstract We investigated global and regional effects of myocardial transplantation of human induced pluripotent stem cell (i PSC )‐derived mesenchymal stem cells (i MSC s) in infarcted myocardium. Acute myocardial infarction ( MI ) was induced by ligation of left coronary artery of severe combined immunodeficient mice before 2 × 10 5 i MSC s or cell‐free saline were injected into peri‐infarcted anterior free wall. Sham‐operated animals received no injection. Global and regional myocardial function was assessed serially at 1‐week and 8‐week by segmental strain analysis by using two dimensional (2D) speckle tracking echocardiography. Early myocardial remodelling was observed at 1‐week and persisted to 8‐week with global contractility of ejection fraction and fractional area change in saline‐ (32.96 ± 14.23%; 21.50 ± 10.07%) and i MSC ‐injected (32.95 ± 10.31%; 21.00 ± 7.11%) groups significantly depressed as compared to sham control (51.17 ± 11.69%, P < 0.05; 34.86 ± 9.82%, P < 0.05). However, myocardial dilatation was observed in saline‐injected animals (4.40 ± 0.62 mm, P < 0.05), but not i MSC s (4.29 ± 0.57 mm), when compared to sham control (3.74 ± 0.32 mm). Furthermore, strain analysis showed significant improved basal anterior wall strain (28.86 ± 8.16%, P < 0.05) in the i MSC group, but not saline‐injected (15.81 ± 13.92%), when compared to sham control (22.18 ± 4.13%). This was corroborated by multi‐segments deterioration of radial strain only in saline‐injected (21.50 ± 5.31%, P < 0.05), but not i MSC (25.67 ± 12.53%), when compared to sham control (34.88 ± 5.77%). Improvements of the myocardial strain coincided with the presence of interconnecting telocytes in interstitial space of the infarcted anterior segment of the heart. Our results show that localized injection of i MSC s alleviates ventricular remodelling, sustains global and regional myocardial strain by paracrine‐driven effect on neoangiogenesis and myocardial deformation/compliance via parenchymal and interstitial cell interactions in the infarcted myocardium.