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Mutations in CBL occur frequently in juvenile myelomonocytic leukemia

2009; Elsevier BV; Volume: 114; Issue: 9 Linguagem: Inglês

10.1182/blood-2009-01-198416

1528-0020

Mignon L. Loh, Debbie S. Sakai, Christian Flotho, Michelle Kang, Manfred Fliegauf, Sophie Archambeault, Charles G. Mullighan, Leslie Chen, Eva Bergstraesser, Carlos E. Bueso‐Ramos, Peter D. Emanuel, Henrik Hasle, Jean‐Pierre J. Issa, Marry M. van den Heuvel‐Eibrink, Franco Locatelli, Jan Starý, M. Trebo, Marcin W. Włodarski, Marco Zecca, Kevin Shannon, Charlotte M. Niemeyer,

Protein Tyrosine Phosphatases

Juvenile myelomonocytic leukemia is an aggressive myeloproliferative disorder characterized by malignant transformation in the hematopoietic stem cell compartment with proliferation of differentiated progeny. Seventy-five percent of patients harbor mutations in the NF1, NRAS, KRAS, or PTPN11 genes, which encode components of Ras signaling networks. Using single nucleotide polymorphism arrays, we identified a region of 11q isodisomy that contains the CBL gene in several JMML samples, and subsequently identified CBL mutations in 27 of 159 JMML samples. Thirteen of these mutations alter codon Y371. In this report, we also demonstrate that CBL and RAS/PTPN11 mutations were mutually exclusive in these patients. Moreover, the exclusivity of CBL mutations with respect to other Ras pathway-associated mutations indicates that CBL may have a role in deregulating this key pathway in JMML.