Portal de Periódicos da CAPES

Conjugal transfer of a small plasmid from Bacillus subtilis to Clostridium acetobutylicum by cointegrate formation with plasmid pAMβ1

1987; Oxford University Press; Volume: 42; Issue: 2-3 Linguagem: Inglês

10.1111/j.1574-6968.1987.tb02056.x

1574-6968

John D. Oultram, A.M.C. Davies, Marian F. Young,

CRISPR and Genetic Engineering

Plasmid pAMβ1 is transferred at low efficiency in filter matings between Bacillus subtilis and Clostridium acetobutylicum. We have constructed a small non-conjugative plasmid, pOD1 (7.2 kbp; ApR, CmR, EmR) that becomes established in pAMβ1-containing strains of B. subtilis in the form of a cointegrate (pAMβ1::pOD1). The cointegrate plasmid is transferred to, and maintained in C. acetobutylicum, with an efficiency similar to that of pAMβ1. We have also constructed derivatives of plasmid pOD1, with single sites for several commonly used restriction endonucleases. They can be used to transfer foreign genes to C. acetobutylicum via B. subtilis.