Petechiae and urticaria after DTP vaccination: Detection of circulating immune complexes containing vaccine-specific antigens
1986; Elsevier BV; Volume: 109; Issue: 6 Linguagem: Inglês
10.1016/s0022-3476(86)80286-4
ISSN1097-6833
AutoresKaren Lewis, Stanley C. Jordan, James D. Cherry, Rebecca Sakai, Chinh T. Lé,
Tópico(s)Immunotherapy and Immune Responses
ResumoCirculating immune complexes are mediators of inflammatory events and have been demonstrated in human diseases such as glomerulonephritis, autoimmune disorders, various malignancies, and certain bacterial, viral, and parasitic infections ~3. CICs are formed by the interaction of soluble antigen with specific antibody in the circulation. They are usually cleared rapidly from the circulation by the reticuloendothelial system. However, when CICs are of a size or quantity that is not adequately cleared, they deposit in vascular beds and activate complement, producing inflammation and tissue injury. Despite ample evidence for the pathogenic potential of CICs and their mediation of many disease entities, little is known about their antigenic composition. Current assays for CICs are not antigen specific; rather, they rely on several immunochemical properties of the CICs (ability to activate complement, and interaction with specific immunochemical probes) for detection. We have recently developed techniques for detection of antigen-specific CICs 4 by utilizing the classic immunochemical principles of Kabat and Heidelberger? Antigen-specific CICs are detected by their ability to be solubilized by preincubation with specific antigen, resulting in diminution in size and number of CICs with reduced binding in the CIC assays. With these techniques, we detected CICs in a 4-
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