The Contractile Potency of Adenosine Triphosphate and Ecto-adenosine Triphosphatase Activity in Guinea Pig Detrusor and Detrusor From Patients With a Stable, Unstable or Obstructed Bladder
2002; Lippincott Williams & Wilkins; Volume: 168; Issue: 3 Linguagem: Inglês
10.1016/s0022-5347(05)64632-0
ISSN1527-3792
AutoresRichard Alan Harvey, Deborah Skennerton, Donald Newgreen, Christopher H. Fry,
Tópico(s)Sexual function and dysfunction studies
ResumoNo AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Sep 2002The Contractile Potency of Adenosine Triphosphate and Ecto-adenosine Triphosphatase Activity in Guinea Pig Detrusor and Detrusor From Patients With a Stable, Unstable or Obstructed Bladder R.A. Harvey, D.E. Skennerton, D. Newgreen, and C.H. Fry R.A. HarveyR.A. Harvey , D.E. SkennertonD.E. Skennerton , D. NewgreenD. Newgreen , and C.H. FryC.H. Fry View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)64632-0AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We compared the potency of adenosine triphosphate (ATP) and its nonhydrolyzable analogue α,β-methylene ATP for generating contractions in human detrusor smooth muscle from patients with a stable, unstable and obstructed bladders. The different ATP potencies were compared with the ecto-adenosine triphosphatase (ATPase) of these samples. Materials and Methods: Contractile experiments were done in vitro by superfusing samples with purines and dose-response curves were generated. Ecto-ATPase activity was measured from the rate of ATP hydrolysis sensitive to the ecto-ATPase inhibitor ARL 67156 with a luciferin-luciferase assay. Results: ATP generated contractions with a mean EC50 of 933 μM. in tissue from stable bladders and was significantly more potent in tissue from unstable and obstructed bladders (EC50 141 and 172 μM., respectively). α,β-methylene ATP was more potent in tissue from stable and unstable bladders (mean combined EC50 3 μM.). In guinea pig detrusor the mean EC50 for ATP and α,β-methylene ATP was 138 and 5.5 μM., respectively. Mean total ATPase activity in unstable bladder biopsies plus or minus standard deviation was about 50% of that in stable bladder biopsies (2.54 ± 1.50 versus 1.37 ± 0.46 nmol. per second−1 per mg. protein−1). The ARL 67156 sensitive fraction was also significantly less in samples from unstable compared with stable bladders (mean 0.94 ± 0.41 versus 0.36 ± 0.26 nmol. per second−1mg. protein−1). Conclusions: The greater potency of ATP for generating contractions in detrusor from unstable bladders may be due to reduced extracellular hydrolysis, allowing purine greater access to detrusor smooth muscle. This finding may explain atropine resistant purine based contractions in detrusor from unstable bladders. References 1 : Evidence for purinergic neurotransmission in human urinary bladder affected by interstitial cystitis. J Urol1993; 150: 2007. Link, Google Scholar 2 : A quantitative study of atropine-resistant contractile in human detrusor smooth muscle, from stable, unstable obstructed bladders. J Urol1999; 162: 1833. Link, Google Scholar 3 : A comparison of the mode of action of ATP carbachol on isolated human detrusor smooth muscle. J Urol1999; 162: 1840. Link, Google Scholar 4 : Effects of cyclopiazonic acid on contractility ecto-ATPase activity in guinea-pig urinary bladder vas deferens. Br J Pharmacol1994; 113: 669. Crossref, Medline, Google Scholar 5 : Enhancement of sympathetic purinergic neurotransmission in the guinea-pig isolated vas deferens by the novel ecto-ATPase inhibitor ARL 67156. Br J Pharmacol1996; 117: 867. Crossref, Medline, Google Scholar 6 : Pharmacological biochemical analysis of FPL 67156, a novel, selective inhibitor of ecto-ATPase. Br J Pharmacol1995; 114: 475. Crossref, Medline, Google Scholar 7 : The ecto-ATPase inhibitor ARL 67156 enhances parasympathetic neurotransmission in the guinea-pig urinary bladder. Eur J Pharmacol1997; 329: 169. Crossref, Medline, Google Scholar 8 : Inhibition of ecto-ATPase by the P2 purinoceptor agonists, ATPgammaS, alpha,beta-methylene-ATP, AMP-PNP, in endothelial cells. Biochem Biophys Res Comm1997; 233: 442. Crossref, Medline, Google Scholar 9 : Functional role of ecto-ATPase activity in goldfish hepatocytes. Am J Physiol1998; 274: R1031. Medline, Google Scholar 10 : Ecto-nucleotidases terminate purinergic signalling in the cochlear endolymphatic compartment. Neuroreport1998; 9: 1559. Medline, Google Scholar 11 : The pharmacology kinetics of ecto-nucleotidases in the perilymphatic compartment of the guinea-pig cochlea. Hearing Res1998; 117: 71. Crossref, Medline, Google Scholar 12 : Effects of adenosine 5′-triphosphate (ATP) beta, gamma-methylene ATP on the rat urinary bladder. Br J Pharmacol1979; 65: 97. Crossref, Medline, Google Scholar 13 : Functional characterization of rat ecto-ATPase ecto-ATP diphosphohydrolase after heterologous expression in CHO cells. Eur J Biochem1999; 262: 102. Crossref, Medline, Google Scholar 14 : Purification characterization of a Ca2+/Mg2+ecto-ATPase from rat heart sarcolemma. Mol Cell Biochem1988; 81: 75. Medline, Google Scholar 15 : Blood flow rate total blood flow related to length density total length of blood vessels in mini-pig urinary bladder after chronic outflow obstruction after recovery from obstruction. Neurourol Urodyn1995; 14: 177. Crossref, Medline, Google Scholar 16 : Evidence for alternative splicing of ecto-ATPase associated with termination of purinergic transmission. Brain Res Mol Brain Res1999; 73: 85. Crossref, Medline, Google Scholar 17 : Properties of proteins associated with the extracellular ATPase of chicken gizzard smooth muscle. A monoclonal antibody study. J Biol Chem1995; 270: 11845. Crossref, Medline, Google Scholar 18 : Molecular cloning expression of a new rat liver cell-CAM105 isoform. Differential phosphorylation of isoforms. Biochem J1992; 285: 47. Crossref, Medline, Google Scholar 19 : Impairment of endothelial subendothelial sites by a circulating plasma factor associated with minimal change disease. Nephrol Dial Transplant1996; 11: 2185. Crossref, Medline, Google Scholar 20 : Evidence for urothelial cell activation in interstitial cystitis. J Urol1993; 149: 470. Link, Google Scholar From the Institute of Urology, London and Discovery Research Pfizer, Sandwich, United Kingdom© 2002 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited by Deng H, Liao L, Li X, Liu Q, Wang X and Zhou Z (2022) Effects of Intravesical Electrical Stimulation on Urinary Adenosine Triphosphate and Nitric Oxide in Rats With Detrusor Underactivity Induced By Bilateral Pelvic Nerve Crush Injury: The Possible Underlying MechanismInternational Neurourology Journal, 10.5213/inj.2244162.081, VOL. 26, NO. 4, (288-298), Online publication date: 30-Dec-2022. Gutierrez Cruz A, Aresta Branco M, Perrino B, Sanders K and Mutafova-Yambolieva V (2022) Urinary ATP Levels Are Controlled by Nucleotidases Released from the Urothelium in a Regulated MannerMetabolites, 10.3390/metabo13010030, VOL. 13, NO. 1, (30) Krishnan A, Sharma G, Devana S, Zohmangaihi D, Mavuduru R, Mandal A, Sharma A and Bora G (2021) Urinary adenosine triphosphate and nitric oxide levels in patients with underactive bladder: a preliminary studyWorld Journal of Urology, 10.1007/s00345-021-03784-5, VOL. 39, NO. 12, (4421-4425), Online publication date: 1-Dec-2021. Kennedy C (2021) ATP as a cotransmitter in sympathetic and parasympathetic nerves - another Burnstock legacyAutonomic Neuroscience, 10.1016/j.autneu.2021.102860, VOL. 235, (102860), Online publication date: 1-Nov-2021. Fry C and McCloskey K (2021) Purinergic signalling in the urinary bladder – When function becomes dysfunctionAutonomic Neuroscience, 10.1016/j.autneu.2021.102852, VOL. 235, (102852), Online publication date: 1-Nov-2021. Silva-Ramos M, Silva I, Faria M, Ferreirinha F and Correia-de-Sá P (2019) Activation of Prejunctional P2x2/3 Heterotrimers by ATP Enhances the Cholinergic Tone in Obstructed Human Urinary BladdersJournal of Pharmacology and Experimental Therapeutics, 10.1124/jpet.119.261610, VOL. 372, NO. 1, (63-72), Online publication date: 1-Jan-2020. McCarthy C, Ikeda Y, Skennerton D, Chakrabarty B, Kanai A, Jabr R and Fry C (2019) Characterisation of nerve‐mediated ATP release from bladder detrusor muscle and its pathological implicationsBritish Journal of Pharmacology, 10.1111/bph.14840, VOL. 176, NO. 24, (4720-4730), Online publication date: 1-Dec-2019. Hao Y, Wang L, Chen H, Hill W, Robson S, Zeidel M and Yu W (2019) Targetable purinergic receptors P2Y12 and A2b antagonistically regulate bladder functionJCI Insight, 10.1172/jci.insight.122112, VOL. 4, NO. 16, Online publication date: 22-Aug-2019. Chakrabarty B, Ito H, Ximenes M, Nishikawa N, Vahabi B, Kanai A, Pickering A, Drake M and Fry C (2019) Influence of sildenafil on the purinergic components of nerve‐mediated and urothelial ATP release from the bladder of normal and spinal cord injured miceBritish Journal of Pharmacology, 10.1111/bph.14669, VOL. 176, NO. 13, (2227-2237), Online publication date: 1-Jul-2019. Antunes-Lopes T and Cruz F (2019) Urinary Biomarkers in Overactive Bladder: Revisiting the Evidence in 2019European Urology Focus, 10.1016/j.euf.2019.06.006, VOL. 5, NO. 3, (329-336), Online publication date: 1-May-2019. Fry C and McCloskey K (2019) Spontaneous Activity and the Urinary Bladder Smooth Muscle Spontaneous Activity, 10.1007/978-981-13-5895-1_5, (121-147), . Fry C and Jabr R (2019) The Integrated Physiology of the Lower Urinary Tract Neurourology, 10.1007/978-94-017-7509-0_7, (65-77), . Iguchi N, Malykhina A and Wilcox D (2018) Early life voiding dysfunction leads to lower urinary tract dysfunction through alteration of muscarinic and purinergic signaling in the bladderAmerican Journal of Physiology-Renal Physiology, 10.1152/ajprenal.00154.2018, VOL. 315, NO. 5, (F1320-F1328), Online publication date: 1-Nov-2018. Sellers D, Chess-Williams R and Michel M (2018) Modulation of lower urinary tract smooth muscle contraction and relaxation by the urotheliumNaunyn-Schmiedeberg's Archives of Pharmacology, 10.1007/s00210-018-1510-8, VOL. 391, NO. 7, (675-694), Online publication date: 1-Jul-2018. Andersson K, Fry C, Panicker J and Rademakers K (2018) Which molecular targets do we need to focus on to improve lower urinary tract dysfunction? ICI-RS 2017Neurourology and Urodynamics, 10.1002/nau.23516, VOL. 37, NO. S4, (S117-S126), Online publication date: 1-Jun-2018. Sana-Ur-Rehman H, Markus I, Moore K, Mansfield K and Liu L (2017) Expression and localization of pannexin-1 and CALHM1 in porcine bladder and their involvement in modulating ATP releaseAmerican Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 10.1152/ajpregu.00039.2016, VOL. 312, NO. 5, (R763-R772), Online publication date: 1-May-2017. Silva-Ramos M, Silva I, Oliveira J and Correia-de-Sá P (2016) Increased Urinary Adenosine Triphosphate in Patients With Bladder Outlet Obstruction Due to Benign Prostate HyperplasiaThe Prostate, 10.1002/pros.23207, VOL. 76, NO. 15, (1353-1363), Online publication date: 1-Nov-2016. Durnin L, Moreland N, Lees A and Mutafova-Yambolieva V (2016) A commonly used ecto-ATPase inhibitor, ARL-67156, blocks degradation of ADP more than the degradation of ATP in murine colonNeurogastroenterology & Motility, 10.1111/nmo.12836, VOL. 28, NO. 9, (1370-1381), Online publication date: 1-Sep-2016. Pakzad M, Ikeda Y, McCarthy C, Kitney D, Jabr R and Fry C (2016) Contractile effects and receptor analysis of adenosine-receptors in human detrusor muscle from stable and neuropathic bladdersNaunyn-Schmiedeberg's Archives of Pharmacology, 10.1007/s00210-016-1255-1, VOL. 389, NO. 8, (921-929), Online publication date: 1-Aug-2016. Kushida N and Fry C (2015) On the origin of spontaneous activity in the bladderBJU International, 10.1111/bju.13240, VOL. 117, NO. 6, (982-992), Online publication date: 1-Jun-2016. Silva-Ramos M, Silva I, Faria M, Magalhães-Cardoso M, Correia J, Ferreirinha F and Correia-de-Sá P (2015) Impairment of ATP hydrolysis decreases adenosine A1 receptor tonus favoring cholinergic nerve hyperactivity in the obstructed human urinary bladderPurinergic Signalling, 10.1007/s11302-015-9478-z, VOL. 11, NO. 4, (595-606), Online publication date: 1-Dec-2015. Silva I, Ferreirinha F, Magalhães-Cardoso M, Silva-Ramos M and Correia-de-Sá P (2015) Activation of P2Y6 Receptors Facilitates Nonneuronal Adenosine Triphosphate and Acetylcholine Release from Urothelium with the Lamina Propria of Men with Bladder Outlet ObstructionJournal of Urology, VOL. 194, NO. 4, (1146-1154), Online publication date: 1-Oct-2015. D׳Agostino G, Maria Condino A and Calvi P (2015) Involvement of β3-adrenoceptors in the inhibitory control of cholinergic activity in human bladder: Direct evidence by [3H]-acetylcholine release experiments in the isolated detrusorEuropean Journal of Pharmacology, 10.1016/j.ejphar.2015.03.074, VOL. 758, (115-122), Online publication date: 1-Jul-2015. White C, Short J, Evans R and Ventura S (2013) Development of a P2X1-purinoceptor mediated contractile response in the aged mouse prostate gland through slowing down of ATP breakdownNeurourology and Urodynamics, 10.1002/nau.22519, VOL. 34, NO. 3, (292-298), Online publication date: 1-Mar-2015. Johal N, Wood D, Wagg A, Cuckow P and Fry C (2014) Functional properties and connective tissue content of pediatric human detrusor muscleAmerican Journal of Physiology-Renal Physiology, 10.1152/ajprenal.00380.2014, VOL. 307, NO. 9, (F1072-F1079), Online publication date: 1-Nov-2014. Mutafova-Yambolieva V and Durnin L (2014) The purinergic neurotransmitter revisited: A single substance or multiple players?Pharmacology & Therapeutics, 10.1016/j.pharmthera.2014.05.012, VOL. 144, NO. 2, (162-191), Online publication date: 1-Nov-2014. Triguero D, Lafuente-Sanchis A and Garcia-Pascual A (2014) Changes in nerve-mediated contractility of the lower urinary tract in a mouse model of premature ageingBritish Journal of Pharmacology, 10.1111/bph.12567, VOL. 171, NO. 7, (1687-1705), Online publication date: 1-Apr-2014. Burnstock G (2013) Purinergic signalling in the urinary tract in health and diseasePurinergic Signalling, 10.1007/s11302-013-9395-y, VOL. 10, NO. 1, (103-155), Online publication date: 1-Mar-2014. Winder M, Tobin G, Zupančič D and Romih R (2014) Signalling Molecules in the UrotheliumBioMed Research International, 10.1155/2014/297295, VOL. 2014, (1-14), . Cheng Y, Mansfield K, Allen W, Chess-Williams R, Burcher E and Moore K (2014) ATP during Early Bladder Stretch Is Important for Urgency in Detrusor Overactivity PatientsBioMed Research International, 10.1155/2014/204604, VOL. 2014, (1-6), . Timóteo M, Carneiro I, Silva I, Noronha-Matos J, Ferreirinha F, Silva-Ramos M and Correia-de-Sá P (2014) ATP released via pannexin-1 hemichannels mediates bladder overactivity triggered by urothelial P2Y6 receptorsBiochemical Pharmacology, 10.1016/j.bcp.2013.11.007, VOL. 87, NO. 2, (371-379), Online publication date: 1-Jan-2014. Elliott R, Tonnu A, Ghaffar N, Taylor A, Tincello D and Norman R (2013) Enhanced purinergic contractile responses and P2X 1 receptor expression in detrusor muscle during cycles of hypoxia-glucopenia and reoxygenation Experimental Physiology, 10.1113/expphysiol.2013.075010, VOL. 98, NO. 12, (1683-1695), Online publication date: 1-Dec-2013. Soler R, Andersson K, Chancellor M, Chapple C, de Groat W, Drake M, Gratzke C, Lee R and Cruz F (2013) Future Direction in Pharmacotherapy for Non-neurogenic Male Lower Urinary Tract SymptomsEuropean Urology, 10.1016/j.eururo.2013.04.042, VOL. 64, NO. 4, (610-621), Online publication date: 1-Oct-2013. Silva-Ramos M, Silva I, Oliveira O, Ferreira S, Reis M, Oliveira J, Correia-de-Sá P and Medeiros R (2013) Urinary ATP May Be a Dynamic Biomarker of Detrusor Overactivity in Women with Overactive Bladder SyndromePLoS ONE, 10.1371/journal.pone.0064696, VOL. 8, NO. 5, (e64696) Walsh C, Cheng Y, Mansfield K, Parkin K, Mukerjee C and Moore K (2012) Decreased Intravesical Adenosine Triphosphate in Patients with Refractory Detrusor Overactivity and BacteriuriaJournal of Urology, VOL. 189, NO. 4, (1383-1387), Online publication date: 1-Apr-2013. Burnstock G (2012) Purinergic signalling in the lower urinary tractActa Physiologica, 10.1111/apha.12012, VOL. 207, NO. 1, (40-52), Online publication date: 1-Jan-2013. Birder L, Ruggieri M, Takeda M, van Koeveringe G, Veltkamp S, Korstanje C, Parsons B and Fry C (2012) How does the urothelium affect bladder function in health and disease?: ICI-RS 2011Neurourology and Urodynamics, 10.1002/nau.22195, VOL. 31, NO. 3, (293-299), Online publication date: 1-Mar-2012. Sacco E (2012) Physiopathology of Overactive Bladder SyndromeUrologia Journal, 10.5301/RU.2012.8972, VOL. 79, NO. 1, (24-35), Online publication date: 1-Jan-2012. D'Agostino G, Condino A, Calvi V, Boschi F, Gioglio L and Barbieri A (2012) Purinergic P2X3 heteroreceptors enhance parasympathetic motor drive in isolated porcine detrusor, a reliable model for development of P2X selective blockers for detrusor hyperactivityPharmacological Research, 10.1016/j.phrs.2011.10.002, VOL. 65, NO. 1, (129-136), Online publication date: 1-Jan-2012. Gomez-Pinilla P, Pozo M and Camello P (2010) Aging differentially modifies agonist-evoked mouse detrusor contraction and calcium signalsAGE, 10.1007/s11357-010-9163-7, VOL. 33, NO. 1, (81-88), Online publication date: 1-Mar-2011. Burnstock G (2011) Therapeutic potential of purinergic signalling for diseases of the urinary tractBJU International, 10.1111/j.1464-410X.2010.09926.x, VOL. 107, NO. 2, (192-204), Online publication date: 1-Jan-2011. Ford A and Cockayne D (2011) ATP and P2X Purinoceptors in Urinary Tract Disorders Urinary Tract, 10.1007/978-3-642-16499-6_22, (485-526), . (2010) Neurological control of pelvic organ functions Pelvic Organ Dysfunction in Neurological Disease, 10.1017/CBO9780511762611.003, (1-60) Santoso A, Sonarno I, Arsad N and Liang W (2010) The Role of the Urothelium and ATP in Mediating Detrusor Smooth Muscle ContractilityUrology, 10.1016/j.urology.2010.06.040, VOL. 76, NO. 5, (1267.e7-1267.e12), Online publication date: 1-Nov-2010. Lawrence G, Aoki K and Dolly J (2010) Excitatory Cholinergic and Purinergic Signaling in Bladder Are Equally Susceptible to Botulinum Neurotoxin A Consistent with Co-Release of Transmitters from Efferent FibersJournal of Pharmacology and Experimental Therapeutics, 10.1124/jpet.110.169342, VOL. 334, NO. 3, (1080-1086), Online publication date: 1-Sep-2010. Fry C (2010) Physiological Properties of the Lower Urinary Tract The Scientific Basis of Urology, Third Edition, 10.3109/9781841847498-16, (244-265), Online publication date: 26-Jul-2010. Fry C, Meng E and Young J (2010) The physiological function of lower urinary tract smooth muscleAutonomic Neuroscience, 10.1016/j.autneu.2009.10.006, VOL. 154, NO. 1-2, (3-13), Online publication date: 1-Apr-2010. Andersson K (2010) Detrusor myocyte activity and afferent signalingNeurourology and Urodynamics, 10.1002/nau.20784, VOL. 29, NO. 1, (97-106), Online publication date: 1-Jan-2010. Roosen A, Chapple C, Dmochowski R, Fowler C, Gratzke C, Roehrborn C, Stief C and Andersson K (2009) A Refocus on the Bladder as the Originator of Storage Lower Urinary Tract Symptoms: A Systematic Review of the Latest LiteratureEuropean Urology, 10.1016/j.eururo.2009.07.044, VOL. 56, NO. 5, (810-820), Online publication date: 1-Nov-2009. Pannek J, Janek S, Sommerer F and Tannapfel A (2008) Expression of purinergic P2X2-receptors in neurogenic bladder dysfunction due to spinal cord injury: a preliminary immunohistochemical studySpinal Cord, 10.1038/sc.2008.165, VOL. 47, NO. 7, (561-564), Online publication date: 1-Jul-2009. Canda A, Chapple C and Chess-Williams R (2009) Pharmacologic responses of the mouse urinary bladderOpen Medicine, 10.2478/s11536-008-0082-2, VOL. 4, NO. 2, (192-197), Online publication date: 1-Jun-2009. Kumcu E, Büyüknacar H, Göçmen C, Evrüke İ and Önder S (2009) Differential effect of neocuproine, a copper(I) chelator, on contractile activity in isolated ovariectomized non-pregnant rat, pregnant rat and pregnant human uterusEuropean Journal of Pharmacology, 10.1016/j.ejphar.2009.01.008, VOL. 605, NO. 1-3, (158-163), Online publication date: 1-Mar-2009. Drake M (2008) Emerging drugs for treatment of overactive bladder and detrusor overactivityExpert Opinion on Emerging Drugs, 10.1517/14728214.13.3.431, VOL. 13, NO. 3, (431-446), Online publication date: 1-Sep-2008. Ballaro A (2008) The Elusive Electromyogram in the Overactive Bladder: A Spark of UnderstandingThe Annals of The Royal College of Surgeons of England, 10.1308/003588408X301217, VOL. 90, NO. 5, (362-367), Online publication date: 1-Jul-2008. Andersson K (2008) PHARMACOLOGIC BASIS OF BLADDER AND URETHRAL FUNCTION AND DYSFUNCTION Female Urology, 10.1016/B978-1-4160-2339-5.50053-7, (47-58), . Chua W, Liu L, Mansfield K, Vaux K, Moore K, Millard R and Burcher E (2007) Age-related changes of P2X1 receptor mRNA in the bladder detrusor from men with and without bladder outlet obstructionExperimental Gerontology, 10.1016/j.exger.2007.02.003, VOL. 42, NO. 7, (686-692), Online publication date: 1-Jul-2007. Burnstock G (2007) Physiology and Pathophysiology of Purinergic NeurotransmissionPhysiological Reviews, 10.1152/physrev.00043.2006, VOL. 87, NO. 2, (659-797), Online publication date: 1-Apr-2007. Ghildyal P, Palani D and Manchanda R (2006) Post- and prejunctional consequences of ecto-ATPase inhibition: electrical and contractile studies in guinea-pig vas deferensThe Journal of Physiology, 10.1113/jphysiol.2006.109678, VOL. 575, NO. 2, (469-480), Online publication date: 1-Sep-2006. Lewis S and Lewis J (2006) Kinetics of urothelial ATP releaseAmerican Journal of Physiology-Renal Physiology, 10.1152/ajprenal.00340.2005, VOL. 291, NO. 2, (F332-F340), Online publication date: 1-Aug-2006. Ruggieri M (2006) Mechanisms of Disease: role of purinergic signaling in the pathophysiology of bladder dysfunctionNature Clinical Practice Urology, 10.1038/ncpuro0456, VOL. 3, NO. 4, (206-215), Online publication date: 1-Apr-2006. Burnstock G (2006) Pathophysiology and Therapeutic Potential of Purinergic SignalingPharmacological Reviews, 10.1124/pr.58.1.5, VOL. 58, NO. 1, (58-86), Online publication date: 1-Mar-2006. Ford A, Gever J, Nunn P, Zhong Y, Cefalu J, Dillon M and Cockayne D (2009) Purinoceptors as therapeutic targets for lower urinary tract dysfunctionBritish Journal of Pharmacology, 10.1038/sj.bjp.0706637, VOL. 147, NO. S2, (S132-S143), Online publication date: 1-Feb-2006. Sun Y and Chai T (2006) Augmented extracellular ATP signaling in bladder urothelial cells from patients with interstitial cystitisAmerican Journal of Physiology-Cell Physiology, 10.1152/ajpcell.00552.2004, VOL. 290, NO. 1, (C27-C34), Online publication date: 1-Jan-2006. Nishiguchi J, Hayashi Y, Chancellor M, de Miguel F, de Groat W, Kumon H and Yoshimura N (2005) Detrusor overactivity induced by intravesical application of adenosine 5′-triphosphate under different delivery conditions in ratsUrology, 10.1016/j.urology.2005.06.099, VOL. 66, NO. 6, (1332-1337), Online publication date: 1-Dec-2005. Rapp D, Lyon M, Bales G and Cook S (2005) A Role for the P2X Receptor in Urinary Tract Physiology and in the Pathophysiology of Urinary DysfunctionEuropean Urology, 10.1016/j.eururo.2005.04.019, VOL. 48, NO. 2, (303-308), Online publication date: 1-Aug-2005. Nyirády P, Thiruchelvam N, Godley M, David A, Cuckow P and Fry C (2005) Contractile properties of the developing fetal sheep bladderNeurourology and Urodynamics, 10.1002/nau.20124, VOL. 24, NO. 3, (276-281), Online publication date: 1-May-2005. Andersson K and Arner A (2004) Urinary Bladder Contraction and Relaxation: Physiology and PathophysiologyPhysiological Reviews, 10.1152/physrev.00038.2003, VOL. 84, NO. 3, (935-986), Online publication date: 1-Jul-2004. Holzer A and Granstein R (2016) Role of Extracellular Adenosine Triphosphate in Human SkinJournal of Cutaneous Medicine and Surgery, 10.1177/120347540400800203, VOL. 8, NO. 2, (90-96), Online publication date: 1-Mar-2004. Dunn J, Ellerkmann R and Bent A (2004) Pathophysiology of Detrusor OveractivityJournal of Pelvic Medicine and Surgery, 10.1097/01.spv.0000128875.25989.80, VOL. 10, NO. 2, (43-51), Online publication date: 1-Mar-2004. Fry C, Ikeda Y, Harvey R, Wu C and Sui G (2004) Control of bladder function by peripheral nerves: avenues for novel drug targetsUrology, 10.1016/j.urology.2003.10.031, VOL. 63, NO. 3, (24-31), Online publication date: 1-Mar-2004. Fry C, Hussain M, McCarthy C, Ikeda Y, Sui G and Wu C (2009) Recent advances in detrusor muscle functionScandinavian Journal of Urology and Nephrology, 10.1080/03008880410015138, VOL. 38, NO. 215, (20-25), Online publication date: 1-Jan-2004. Burnstock G and Knight G (2004) Cellular Distribution and Functions of P2 Receptor Subtypes in Different Systems , 10.1016/S0074-7696(04)40002-3, (31-304), . Andersson K (2003) Storage and voiding symptoms: pathophysiologic aspectsUrology, 10.1016/j.urology.2003.09.030, VOL. 62, NO. 5, (3-10), Online publication date: 1-Nov-2003. Thiruchelvam N, Wu C, David A, Woolf A, Cuckow P and Fry C (2003) Neurotransmission and viscoelasticity in the ovine fetal bladder after in utero bladder outflow obstructionAmerican Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 10.1152/ajpregu.00688.2002, VOL. 284, NO. 5, (R1296-R1305), Online publication date: 1-May-2003. Volume 168Issue 3September 2002Page: 1235-1239 Advertisement Copyright & Permissions© 2002 by American Urological Association, Inc.Keywordsbladdermuscle, smoothguinea pigsadenosine triphosphatebladder neck obstructionMetrics Author Information R.A. Harvey More articles by this author D.E. Skennerton More articles by this author D. Newgreen More articles by this author C.H. Fry More articles by this author Expand All Advertisement PDF downloadLoading ...
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