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Highly stable colorimetric aptamer sensors for detection of ochratoxin A through optimizing the sequence with the covalent conjugation of hemin

2014; Royal Society of Chemistry; Volume: 139; Issue: 7 Linguagem: Inglês

10.1039/c3an01639k

1364-5528

Jayeon Lee, Chang Hoon Jeon, Sang Jung Ahn, Tai Hwan Ha,

Molecular Sensors and Ion Detection

Optimization of hairpin DNA is introduced to detect ochratoxin A (OTA) by chemically conjugating its cofactor, hemin, toward the 5'-end. The newly designed OTA aptasensor showed enhanced stability and sensitivity, thereby lowering the detection limit to an ~1 nM level. Furthermore, an optimal spacer for hemin conjugation was investigated for stable responses toward very diluted OTA solutions.