Production scale purification of biosynthetic human insulin by reversed-phase high-performance liquid chromatography
1989; Elsevier BV; Volume: 461; Linguagem: Inglês
10.1016/s0021-9673(00)94274-2
ISSN1873-3778
AutoresEugene P. Kroef, Rebecca A. Owens, Eddie L. Campbell, Ronald D. Johnson, Harlene I. Marks,
Tópico(s)Crystallization and Solubility Studies
ResumoA process based on reversed-phase high-performance liquid chromatography (RP-HPLC) has been developed for the purification of biosynthetic human insulin (BHI). The RP-HPLC procedure has been successfully integrated into the multimodal chromatographic production process used to purify kilogram quantities of BHI. Axial compression column technology was used in the scale-up process. The RP-HPLC procedure yields an insulin product having high chemical purity and full biological activity.
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