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NOVEL DESIGN FOR CENTRIFUGAL COUNTER-CURRENT CHROMATOGRAPHY: III. SAW TOOTH COLUMN

2010; Taylor & Francis; Volume: 33; Issue: 6 Linguagem: Inglês

10.1080/10826071003684547

1520-572X

Yi Yang, Haji Akber Aisa, Yoichiro Ito,

Natural product bioactivities and synthesis

The toroidal coil using an equilateral triangular core and zigzag pattern column have improved both retention of the stationary phase and peak resolution of the conventional toroidal coil in centrifugal counter-current chromatography. To further improve the retention of stationary phase and peak resolution, a novel saw tooth column was designed and the performance of the system was evaluated at various flow rates. The results indicated that both retention of the stationary phase and peak resolution were improved as the flow rate was decreased and at a flow rate of 0.005 mL/min the resolution is remarkably increased. Modification of the tubing called flat-twisted tubing further improved the peak resolution without increasing the column pressure. With decreased column length at a capacity of about 0.2 mL, resolution of the saw tooth column was 1.02.