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A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4

2011; Instituto de Tecnologia do Paraná (Tecpar); Volume: 54; Issue: 6 Linguagem: Inglês

10.1590/s1516-89132011000600013

1678-4324

Francisca Acevedo, Leticia Pizzul, María del Pilar Castillo, Olga Rubilar, María Elena Lienqueo, Gonzalo Tortella, M.C. Díez,

Algal biology and biofuel production

In this study, different growth conditions of Anthracophyllum discolor Sp4 including the effect of agitation, additions of lignocellulosic support, inducer and surfactant were evaluated on the MnP production in Kirk medium using a culture system made up of the tubes containing the glass bead . The highest MnP production (1,354 U/L on day 13) was obtained when the medium was supplemented with wheat grain and 0.25 mM MnSO4 as inducer, under static conditions at 30°C. Two isoenzymes were purified (35 and 38 kDa respectively). MnP presented a maximal activity in the pH range between 4.5 and 5.5, a relatively high temperature tolerance (50ºC) and a high catalytic activity for 2,6-dimethoxyphenol and hydrogen peroxide.