Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the ( E )‐4‐hydroxy‐3‐methylbut‐2‐enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe–4S] protein
2003; Wiley; Volume: 541; Issue: 1-3 Linguagem: Inglês
10.1016/s0014-5793(03)00317-x
ISSN1873-3468
AutoresMurielle Wolff, Myriam Seemann, Bernadette Tse Sum Bui, Yves‐Michel Frapart, Denis Tritsch, Ana Garcia Estrabot, Manuel Rodríguez‐Concepción, Albert Boronat, Andrée Marquet, Michel Rohmer,
Tópico(s)Antioxidant Activity and Oxidative Stress
ResumoThe last enzyme (LytB) of the methylerythritol phosphate pathway for isoprenoid biosynthesis catalyzes the reduction of ( E )‐4‐hydroxy‐3‐methylbut‐2‐enyl diphosphate into isopentenyl diphosphate and dimethylallyl diphosphate. This enzyme possesses a dioxygen‐sensitive [4Fe–4S] cluster. This prosthetic group was characterized in the Escherichia coli enzyme by UV/visible and electron paramagnetic resonance spectroscopy after reconstitution of the purified protein. Enzymatic activity required the presence of a reducing system such as flavodoxin/flavodoxin reductase/reduced nicotinamide adenine dinucleotide phosphate or the photoreduced deazaflavin radical.
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