Resolution and concentration detection limit in capillary gel electrophoresis
1991; Elsevier BV; Volume: 545; Issue: 1 Linguagem: Inglês
10.1016/s0021-9673(01)88705-7
ISSN1873-3778
AutoresJán Macek, U. R. Tjaden, J. van der Greef,
Tópico(s)Electrowetting and Microfluidic Technologies
ResumoThe effects of column length and applied electric field on the column efficiency and the resolution of two oligonucleotides in capillary gel electrophoresis were examined. The best resolution was obtained by using long columns in combination with optimum (and not the highest possible) electric field. Further, the concentration detection limits were optimized; it was found that electrokinetic injection did not cause excessive peak broadening even when applied for 25 s at the operating voltage. Thus sample molecules were concentrated during the injection by isotachophoretic effects resulting in very low concentration detection limits (216 pmol/l for a dodecanucleotide with a relative molecular mass of 3707).
Referência(s)