Identification and quantification of phytochemicals in nutraceutical products from green tea by UHPLC–Orbitrap-MS
2014; Elsevier BV; Volume: 173; Linguagem: Inglês
10.1016/j.foodchem.2014.10.092
ISSN1873-7072
AutoresNoelia López-Gutiérrez, Roberto Romero‐González, Patricia Plaza‐Bolaños, José Luis Martı́nez Vidal, Antonia Garrido Frenich,
Tópico(s)Phytoestrogen effects and research
ResumoA method has been developed and validated for the simultaneous detection and quantification of phytochemicals in nutraceutical products obtained from green tea. For that purpose, ultra-high performance liquid chromatography coupled to single-stage Orbitrap high resolution mass spectrometry (UHPLC–Orbitrap-MS) has been used. A database containing 37 compounds has been used for the detection and identification of the target compounds. The developed methodology was based on solid–liquid extraction, using a mixture of methanol:H2O (80:20, v/v, pH 4), followed by dilution (10 times) with a mixture of ammonium acetate:methanol (50:50, v/v). Chromatographic conditions were optimised and full scan accurate mass data acquisition using electrospray ionisation in positive and negative ion mode was used. Moreover, all-ion fragmentation mode was used to get information of fragment ions, and they were used for identification purposes. The developed method was validated, obtaining repeatability (intra-day) and inter-day precision values (expressed as relative standard deviation, RSD) lower than 16% and 20%, respectively. Lower limits were also evaluated and limits of detection (LODs) ranged from 1 to 50 μg L−1, while limits of quantification (LOQs) ranged from 2 to 150 μg L−1. Recovery was performed at five levels and it ranged from 70% to 109%. Finally, this method was used to evaluate the phytochemical content in 10 samples (tablets or capsules), showing concentrations of (+)-catechin, (−)-epicatechin, gallic acid, (−)-gallocatechin and quercetin-3-O-rutinoside, ranging from 258 (C6) to 10,729 (C6) mg kg−1.
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