Ligation of a Synthetic Peptide to the N Terminus of a Recombinant Protein Using Semisynthetic Protein trans ‐Splicing
2006; Wiley; Volume: 45; Issue: 31 Linguagem: Inglês
10.1002/anie.200600570
ISSN1521-3773
AutoresChristina Ludwig, Martina Pfeiff, Uwe Linne, Henning D. Mootz,
Tópico(s)Biochemical and Structural Characterization
ResumoSplice it! An active intein can be reconstituted from a recombinant C-terminal and a short synthetic N-terminal fragment. This allows ligation of a synthetic peptide to the N terminus of a recombinant protein of interest by protein trans-splicing and circumvents the requirement for a C-terminal thioester or other reactive groups common to chemical ligation approaches. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z600570_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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