Autoradiographic study of degenerative and regenerative proliferation of neuroglia cells with tritiated thymidine
1962; Elsevier BV; Volume: 5; Issue: 4 Linguagem: Inglês
10.1016/0014-4886(62)90040-7
ISSN1090-2430
Autores Tópico(s)Barrier Structure and Function Studies
ResumoProliferation of neuroglia cells was provoked in rats by bilateral electrolytic lesions in the lateral geniculate body and this was combined with unilateral intracranial injection of thymidine-H3. The animals were then sacrificed 1 day, 1 week, 2 weeks, 1 month, and 2 months after the operation. Using fine-resolution autoradiography, reduced silver grains were concentrated over nuclei of neuroglia and microglia cells in and around the lesion area, and in various visual structures not directly affected by the lesion, such as the optic radiation, lower layers of the striate cortex, the pretectal region, and the brachium of the superior colliculus. Labeled neuroglia cells were also seen in structures which do not obtain fibers directly from the lateral geniculate body, such as the corpus callosum and hippocampus. Labeled neuroglia cells in all these structures were present on both the injected and noninjected side. The concentration of grains over neuroglia cells was highest in animals with 1-day survival period, and then declined, while the number of labeled cells was lowest after 1 day of survival and then increased up to 1 month—suggesting continued division of the already labeled cells. The experiment suggested that not only astrocytes and microglia cells, but also oligodendrocytes proliferate. Astrocytes and microglia cells predominated in the directly affected regions, while oligodendrocytes were most numerous along the pathways and gray matter which are directly or indirectly connected with the lateral geniculate body.
Referência(s)