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A premutational state induced in yeast by ethidium bromide

1971; Elsevier BV; Volume: 44; Issue: 2 Linguagem: Inglês

10.1016/0006-291x(71)90593-6

1090-2104

Philip S. Perlman, Henry R. Mahler,

RNA and protein synthesis mechanisms

Ethidium Bromide (EB) has been known to be an effective and irreversible mutagen in converting wild-type (ϱ+) yeast cells to the cytoplasmic petite (ϱ−) state. The effects of EB may be reversed by incubation of treated cells in phosphate buffer at elevated temperatures (≥ 42°). The temperature dependence of mutagenesis is different from that of reversal. It is proposed that reversal is related to a heat-induced alteration in a membrane-mtDNA-EB complex.