Immunogenicity of peptides derived from a fibronectin-binding protein of S. aureus expressed on two different plant viruses
1999; Elsevier BV; Volume: 17; Issue: 15-16 Linguagem: Inglês
10.1016/s0264-410x(98)00485-x
ISSN1873-2518
AutoresFrank R. Brennan, Timothy D. Jones, Marian Longstaff, Sean Chapman, Trevor Bellaby, Heather Smith, Fengfeng Xu, W D Hamilton, Jan‐Ingmar Flock,
Tópico(s)Bacteriophages and microbial interactions
ResumoThe D2 peptide derived from an S. aureus fibronectin-binding protein (FnBP) was expressed on the surface of the icosahedral cowpea mosaic virus (amino acids 1–30 of D2) or on the rod-shaped potato virus X (amino acids 1–38 of D2), termed CPMV-MAST1 and PVX-MAST8, respectively. Mice and rats were immunized subcutaneously with CPMV-MAST1 and mice with PVX-MAST8 in adjuvant and high titres of FnBP-specific antibody were obtained. The mouse IgG was predominantly of the IgG2a and IgG2b isotypes, which strongly bound complement component C1q, suggesting a TH1-bias in the peptide-specific responses. Sera from mice and rats immunized with CPMV-MAST1 and from mice immunized with PVX-MAST8 were shown to completely inhibit the binding of fibronectin to immobilised recombinant FnBP and rat sera against CPMV-MAST1 were able to block adherence of S. aureus to fibronectin. These studies demonstrate that the D2 peptide is highly immunogenic when expressed on 2 different plant viruses and highlight the potential of plant virus-based vaccines to protect against S. aureus infections.
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