Detection of <i>Chlamydia trachomatis</i> in Pregnant Women by the Papanicolaou Technique, Enzyme Immunoassay and Polymerase Chain Reaction
2000; Karger Publishers; Volume: 44; Issue: 2 Linguagem: Inglês
10.1159/000326347
ISSN1938-2650
AutoresCarmen Adriana Bañuelos Pánuco, Irma Deleón Rodríguez, José Tomás Hernández Méndez, Lydia Alejandra Martı́nez Guzmán, David Akle Fierro, Juan Murillo, Elba Reyes Maldonado,
Tópico(s)Urinary and Genital Oncology Studies
ResumoTo compare Papanicolaou staining, enzyme immunoassay (EIA) and the polymerase chain reaction (PCR) techniques for detecting Chlamydia trachomatis in pregnant women.Endocervical specimens were taken randomly from 125 pregnant women with or without symptoms. These women attended their first medical consultation at the Regional General Ignacio Zaragoza Hospital. Samples were analyzed for detection of C trachomatis. When results differed between tests, specimens were evaluated by direct immunofluorescence staining.The prevalence of chlamydial infection was 2.4%. The characteristics of patients positive for Chlamydia were: average age, 24 years; first sexual encounter at age 21 years, one partner and six to nine months of gestation. The sensitivity, specificity, accuracy, positive predictive values and negative predictive values were 100%, 99.18%, 99.20%, 75% and 100%, respectively, for Papanicolaou staining; 100%, 92.62%, 92%, 25% and 100% for EIA; and 100%, 100%, 100% and 100% for PCR.Both Papanicolaou staining and PCR were adequate for diagnosis of C trachomatis infection. EIA was not reliable and therefore is not recommended for use as a diagnostic technique in a pregnant population with low risk and low prevalence.
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