Analysis of myeloid characteristics in acute lymphoblastic leukemia
1993; Elsevier BV; Volume: 17; Issue: 9 Linguagem: Inglês
10.1016/0145-2126(93)90116-3
ISSN1873-5835
AutoresHiroyuki Tsuchiya, Soheir S. El-Sonbaty, Masahiko Watanabe, Hitoshi Suzushima, Norio Asou, Tetsuji Murakami, Takeo Takeda, Akihiro Shimosaka, Kiyoshi Takatsuki, Ichiro Matsuda,
Tópico(s)Chronic Myeloid Leukemia Treatments
ResumoWe examined myeloid characteristics of myeloid-antigen-positive (My+) and -negative (My−) B-precursor acute lymphoblastic leukemia (ALL) blast cells. Immunophenotyping before and after culture, rearrangements of immunoglobulin heavy chain (IgH) and T-cell receptor (TCR) genes, stimulation of DNA synthesis with granulocyte colony-stimulating factor (G-CSF), and G-CSF binding assay were performed. Of My+ ALL blasts, the immunophenotypic staging as B-precursor ALL and rearrangements of IgH and TCR-β, γ and δ genes did not differ from findings in My− ALL blasts. Stimulated with G-CSF, cells from one My+ ALL and from one My− ALL patients showed enhancement of DNA synthesis and expression of CD11b and CD13, respectively. G-CSF binding was observed in blasts from 3 My+ ALL patients and one My− ALL child. After culture, blasts from My− ALL children expressed CD13 but showed neither enhanced DNA synthesis with G-CSF nor G-CSF binding. Thus, it would appear that (i) My+ and My− adult ALL blasts are at the same stage of differentiation; (ii) some My+ adult ALL blasts have phenotypic and functional myeloid characteristics; and (iii) induction of CD13 expression in My− ALL after in vitro culture does not correlate with other myeloid characteristics.
Referência(s)