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Human hepatic cell uptake of resveratrol: involvement of both passive diffusion and carrier-mediated process

2004; Elsevier BV; Volume: 316; Issue: 4 Linguagem: Inglês

10.1016/j.bbrc.2004.02.164

1090-2104

Allan Lançon, D Delma, Hanan Osman‐Ponchet, J. P. Thenot, N Latruffe B.Jannin,

RNA Interference and Gene Delivery

This work reports significant advances on the transport in hepatic cells of resveratrol, a natural polyphenol with potent protective properties. First, we describe a new simple technique to qualitatively follow resveratrol cell uptake and intracellular distribution, based on resveratrol fluorescent properties. Second, the time-course study and the quantification of 3H-labelled resveratrol uptake have been performed using human hepatic derived cells (HepG2 tumor cells) and hepatocytes. The temperature-dependence of the kinetics of uptake as well as the cis-inhibition experiments agree with the involvement of a carrier-mediated transport in addition to passive diffusion. The decrease of passive uptake resulting from resveratrol binding to serum proteins brings to light a mediated mechanism in physiological situation.