Functional Domains of Rts1 and P1 RepA Proteins for Initiation of Replication
1998; Elsevier BV; Volume: 40; Issue: 2 Linguagem: Inglês
10.1006/plas.1998.1360
ISSN1095-9890
AutoresYong Fang Li, Tetsuya Hayashi, Yoshiro Terawaki,
Tópico(s)Enzyme Structure and Function
ResumoRts1 RepA and P1 RepA aretrans-acting proteins essential for initiation of replication of Rts1 and P1 plasmids, respectively. We recently found that P1 RepA boundin vitroto the Rts1 replication origin as strongly as Rts1 RepA and activated the originin vivo.However, theoriactivation was quite inefficient. This study shows that by replacing a small region of P1 RepA with the corresponding region of Rts1 RepA, the efficiency of Rts1oriactivation increased markedly. Interestingly, the same subregion of P1 RepA was found to be important forin vivoactivation of the P1 origin. Thus, a region essential for efficient activation of the replication origin was assigned to the P1 RepA molecule as well as to the Rts1 RepA molecule. The region was distinct from a domain necessary forin vitrobinding to the origin, although both regions were required forin vivoactivation of the respective origin.
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