Radioimmunotherapy with Alpha-Particle-Emitting Immunoconjugates
1988; American Association for the Advancement of Science; Volume: 240; Issue: 4855 Linguagem: Inglês
10.1126/science.2897133
ISSN1095-9203
AutoresRoger M. Macklis, Berma M. Kinsey, Amin I. Kassis, James L.M. Ferrara, Robert W. Atcher, J. Hines, C. Norman Coleman, S. James Adelstein, Steven J. Burakoff,
Tópico(s)Immunotherapy and Immune Responses
ResumoAlpha particles are energetic short-range ions whose higher linear energy transfer produces extreme cytotoxicity. An α-particle-emitting radioimmunoconjugate consisting of a bismuth-212-labeled monoclonal immunoglobulin M specific for the murine T cell/neuroectodermal surface antigen Thy 1.2 was prepared. Analysis in vitro showed that the radioimmunoconjugate was selectively cytotoxic to a Thy 1.2 + EL-4 murine tumor cell line. Approximately three bismuth-212-labeled immunoconjugates per target cell reduced the uptake of [ 3 H]thymidine by the EL-4 target cells to background levels. Mice inoculated intraperitoneally with EL-4 cells were cured of their ascites after intraperitoneal injection of 150 microcuries of the antigen-specific radioimmunoconjugate, suggesting a possible role for such conjugates in intracavitary cancer therapy.
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