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Identification of the glycosaminoglycan keratan sulfate in the prostatic secretory cell

2000; Wiley; Volume: 44; Issue: 3 Linguagem: Inglês

10.1002/1097-0045(20000801)44

1097-0045

Ronald J. Cohen, John W. Holland, Sharon L. Redmond, John E. McNeal, Hugh Dawkins,

Glycosylation and Glycoproteins Research

The ProstateVolume 44, Issue 3 p. 204-209 Identification of the glycosaminoglycan keratan sulfate in the prostatic secretory cell Ronald J. Cohen, Corresponding Author Ronald J. Cohen ronnie@urc.urc.uwa.edu.au Urological Research Centre, Department of Surgery, University of Western Australia, Perth, Western Australia, AustraliaUrological Research Centre, Level 2, M Block, Queen Elizabeth II Medical Centre, Nedlands 6009, Western Australia, AustraliaSearch for more papers by this authorJohn W. Holland, John W. Holland Urological Research Centre, Department of Surgery, University of Western Australia, Perth, Western Australia, AustraliaSearch for more papers by this authorSharon L. Redmond, Sharon L. Redmond Urological Research Centre, Department of Surgery, University of Western Australia, Perth, Western Australia, AustraliaSearch for more papers by this authorJohn E. McNeal, John E. McNeal Department of Urology, Stanford University Medical Center, Stanford, CaliforniaSearch for more papers by this authorHugh J.S. Dawkins, Hugh J.S. Dawkins Urological Research Centre, Department of Surgery, University of Western Australia, Perth, Western Australia, AustraliaSearch for more papers by this author Ronald J. Cohen, Corresponding Author Ronald J. Cohen ronnie@urc.urc.uwa.edu.au Urological Research Centre, Department of Surgery, University of Western Australia, Perth, Western Australia, AustraliaUrological Research Centre, Level 2, M Block, Queen Elizabeth II Medical Centre, Nedlands 6009, Western Australia, AustraliaSearch for more papers by this authorJohn W. Holland, John W. Holland Urological Research Centre, Department of Surgery, University of Western Australia, Perth, Western Australia, AustraliaSearch for more papers by this authorSharon L. Redmond, Sharon L. Redmond Urological Research Centre, Department of Surgery, University of Western Australia, Perth, Western Australia, AustraliaSearch for more papers by this authorJohn E. McNeal, John E. McNeal Department of Urology, Stanford University Medical Center, Stanford, CaliforniaSearch for more papers by this authorHugh J.S. Dawkins, Hugh J.S. Dawkins Urological Research Centre, Department of Surgery, University of Western Australia, Perth, Western Australia, AustraliaSearch for more papers by this author First published: 17 July 2000 https://doi.org/10.1002/1097-0045(20000801)44:3 3.0.CO;2-WCitations: 8AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract BACKGROUND Prostate secretory granules (PSG) represent the basic secretory unit of the prostate gland, containing many of its exocrine proteases. Recent analysis of intraluminal corpora amylacea, a proposed by-product of PSG secretion, detected sulfated glycosaminoglycans (GAG) possibly keratan sulfate (KS), indicating a secretory mechanism for GAG in the human prostate surface epithelial cell. METHODS Immunostains using anti-KS and anti-prostate-specific antigen (PSA) were evaluated on 10 sequential radical prostatectomy specimens, three of which had received neoadjuvant antiandrogen therapy. Extracts of normal secretory tissue as well as a sample composed almost entirely of prostatic stroma were subjected to Western blot analysis, using the same antibody panel. RESULTS Keratan sulfate secretion from the normal prostate epithelial cell has been confirmed and correlates, as does PSA, with the presence of cytoplasmic PSG. No such correlation exists in most adenocarcinomas or in benign epithelium after androgen ablation. Western blot analyses confirmed tissue immunostains and demonstrated a secretory proteoglycan of 70–95 kDa. CONCLUSIONS Recognition of PSG heralds a novel secretory mechanism within the human prostate gland that is linked to the secretion of KS. The role of KS in normal prostate secretion remains unknown, although it appears downregulated in neoplastic and androgen-ablated cells. Prostate 44:204–209, 2000. © 2000 Wiley-Liss, Inc. Citing Literature Volume44, Issue31 August 2000Pages 204-209 RelatedInformation