High-performance thin-layer chromatography–bioautography for multiple antibiotic residues in cow’s milk
2003; Elsevier BV; Volume: 784; Issue: 2 Linguagem: Inglês
10.1016/s1570-0232(02)00819-x
ISSN1873-376X
AutoresA. Ramírez, Rey Gutiérrez Tolentino, Gilbert Diaz, Carlos González, Norma Pérez, S Vega, M Noa,
Tópico(s)Pharmaceutical and Antibiotic Environmental Impacts
ResumoAn analytical method to identify and quantify multiple antibiotic residues (chloramphenicol, ampicillin, benzylpenicillin, dicloxacillin and erythromycin) in cow's milk by high-performance thin-layer chromatography (HPTLC) combined with bioautography was developed. The test microorganism used for bioautography was Bacillus subtilis ATCC 6633. Antibiotic residues were extracted with acetonitrile, fat eliminated with petroleum ether and residues isolated with dichloromethane The sensitivity of the method guarantees the detection of the above-mentioned antibiotics at levels below maximum residue limits (MRL) allowed for milk. Percentage recoveries ranged between 90 and 100%, with coefficients of variation between 7.2 and 21.3%. Some advantages of this methodology over thin-layer chromatography (TLC)/bioautography are also discussed.
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