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Immobilization of pneumococcal polysaccharide vaccine on silicon oxide wafer for an acoustical biosensor

2000; Elsevier BV; Volume: 15; Issue: 9-10 Linguagem: Inglês

10.1016/s0956-5663(00)00084-1

1873-4235

Rosa F. Dutra, Cesar M. Castro, Christophe Azevedo, Ester Vinhas, E Malagueño, E. H. M. Melo, J. L. Lima Filho, John F. Kennedy,

Biosensors and Analytical Detection

One the most important aspects of a biosensor is related to immobilization and maintenance of specific reference compounds on sensing surfaces. A method for the immobilization of polysaccharides to a silicon oxide surface intended for Surface Acoustical Waves (SAW) sensors is described. Silicon oxide is a hydrophobic inorganic support used for the fabrication of many electronic devices. The pneumococcal polysaccharide (PPS) vaccine is immobilized via Protein A after pre-treatment of the surface with hydrochloric acid. The effects of non-specific binding are discussed. The results indicate that the immobilization of PPS via Protein A increases the sensitivity of detecting Streptococcus pneumoniae antibodies in human sera and offers greater reproducibility of response compared with ELISA methods. The principles of this technique are simple and are applicable to the immobilization of many capsular polysaccharides.