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A Fast Made Disposable Glucose Biosensor Incorporating the Oxidase and a Ferrocene in an Alginate Gel

1994; Taylor & Francis; Volume: 27; Issue: 15 Linguagem: Inglês

10.1080/00032719408000294

1532-236X

Alexander D. Ryabov, Marina Ivanova, Boris B. Gnedenko, R. Valunas§,

Analytical Chemistry and Sensors

Abstract A simple procedure is described for co-immobilization of glucose oxidase and dimethylaminomethylferrocene in a sodium alginate gel on the surface of pyrolytic graphite electrode. The film is electrochemically active and the peak current is a function of D-glucose concentration with the apparent Michaelis-Menten constant Km = (1.2±0.3) × 10−2 M. The optimal concentration range for biosensoric applications in 0.001–0.010 M.