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BIBTEX RIS

Methodological variations in the isolation of genomic DNA from Streptococcus bacteria

2010; Instituto de Tecnologia do Paraná (Tecpar); Volume: 53; Issue: 4 Linguagem: Inglês

10.1590/s1516-89132010000400013

ISSN

1678-4324

Autores

Mônica Moreira, Juliana Noschang, Ivana Froede Neiva, Yanê Carvalho, llma Hiroko Higuti, Vânia Aparecida Vicente,

Tópico(s)

Genomics and Phylogenetic Studies

Resumo

In this work, genomic DNA of Streptococcus pyogenes, S. mutans and S. sobrinus was isolated using two methods: either using the detergent cetyltrimethylammonium bromide (CTAB) at 65ºC; or by applying ultrasound to a mixture of silica and celite in CTAB. The composite method that used ultrasound was the more efficient, allowing the straightforward extraction of genomic DNA from Gram-positive bacteria with good quality and reproducibility.

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