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The use of Armored RNA as a multi-purpose internal control for RT-PCR

2008; Elsevier BV; Volume: 150; Issue: 1-2 Linguagem: Inglês

10.1016/j.jviromet.2008.02.007

1879-0984

Jeffery Stevenson, Weston Hymas, David R. Hillyard,

Bacteriophages and microbial interactions

Real time reverse transcriptase-PCR (RT-PCR) is now used commonly for the detection of viral pathogens in respiratory samples. However, due to potential inhibition of the RT-PCR or inefficient extraction, this sample type can present significant challenges to accurate patient testing. The goal of this study was to create an internal control to be multiplexed in a real time RT-PCR assay for detecting a viral target in respiratory samples. This report describes an Armored RNA (aRNA) internal control developed originally to be multiplexed in a real time RT-PCR assay for detecting SARS-associated Coronavirus, but can be incorporated into any RT-PCR assay. The internal control primers and probe target a region in the coat protein gene of the E. coli F-specific bacteriophage ms2, which is contained within the aRNA.