Clonal Expansion of T Cells That Are Specific for Autologous Ovarian Tumor among Tumor-Infiltrating T Cells in Humans1
1999; Elsevier BV; Volume: 74; Issue: 1 Linguagem: Inglês
10.1006/gyno.1999.5430
ISSN1095-6859
AutoresKazuhiko Hayashi, Kyoko Yonamine, Kayo Masuko, Tomohiro Iida, Kazuhiko Yamamoto, Kusuki Nishioka, Tomohiro Kato,
Tópico(s)Immune Cell Function and Interaction
ResumoAbstract Objective. The purpose of this study was to determine whether oligoclonally expanding tumor-infiltrating lymphocytes (TIL) were tumor-specific. Study design. Peripheral blood lymphocytes (PBL) from an ovarian tumor-bearing patient were stimulated in vitro with an autologous cancer cell line (SMOV-2). Then genes coding for the third complementarity-determining region of the T cell receptor (TCR) β chain were amplified by reverse transcription-polymerase chain reaction and separated by single-strand conformation polymorphism. Accumulated TCR clonotypes in vitro and in vivo in TIL were compared. Results. Clonal expansion of T cells was generated from PBL by stimulation with SMOV-2. A portion of the proliferated clonotypes was found to be identical to those accumulated in TIL in vivo. Conclusion. This is the first demonstration that accumulating T cell clones in TIL recognize antigen(s) on an autologous tumor. Further characterization of such T cell clonotypes may lead to tumor antigen-specific immunotherapy.
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