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Copy Number Differences of Y Chromosomal Genes Between Superior and Inferior Quality Semen Producing Crossbred ( Bos taurus × Bos indicus ) Bulls

2014; Taylor & Francis; Volume: 26; Issue: 1 Linguagem: Inglês

10.1080/10495398.2014.887020

1532-2378

Ayan Mukherjee, Gulshan Dass, Jagan Mohanarao G., Vinay Kumar Katneni, Dipak Banerjee, Tapan Kumar Das, Moloya Gohain, A. K. Chakrabarty, Tirtha Kumar Datta, Sachinandan De,

Genetic and phenotypic traits in livestock

Abstract The removal of crossbred bulls from semen collection programs due to the production of poor quality semen causes substantial monetary losses to the dairy industry. Seminal quality, a quantitative trait, is greatly influenced by genome level variations. Deletion and/or duplication of Y chromosomal genes and subsequent changes in gene copy number have a major role in determining spermatogenic efficiency and, therefore, seminal quality. In this study, copy numbers of three Y chromosomal genes TSPY, DDX3Y, and USP9Y in genomic DNA were estimated and compared in two groups of crossbred (Bos taurus × Bos indicus) bulls of ten each, superior and inferior quality semen producing bulls, which were classified based on their seminal quality parameters. For TSPY gene, the inferior quality semen donor group has significantly lower copy number than superior quality semen donor group (p < 0.05). No significant difference was found in DDX3Y and USP9Y gene copy numbers between two groups (p > 0.05). In conclusion, this study demonstrates that the copy number of TSPY, a Y chromosomal spermatogenesis related gene, may be an important determinant to predict the quality of bull semen, facilitating better selection of bulls in a herd for semen collection program. Keywords: Absolute copy numberCrossbred bull DDX3Y gene TSPY gene USP9Y geneY chromosome ACKNOWLEDGMENT The authors thank the Director, National Dairy Research Institute, Karnal, India for all the necessary facilities during the course of the study. Notes a Number of cells/reaction genomic DNA. b Number of USP9Y copies/reaction genomic DNA. c Number of DDX3Y copies/reaction genomic DNA. d Number of TSPY copies/reaction genomic DNA. Color versions of one or more of the figures in the article can be found online at www.tandfonline.com/labt.