Development and validation of a sandwich ELISA for quantification of peanut agglutinin (PNA) in foods
2011; Taylor & Francis; Volume: 23; Issue: 3 Linguagem: Inglês
10.1080/09540105.2011.617358
ISSN1465-3443
AutoresXiaofang Deng, Liqiang Liu, Wenwei Ma, Chuanlai Xu, Libing Wang, Hua Kuang,
Tópico(s)RNA and protein synthesis mechanisms
ResumoAbstract In this study, two anti-peanut agglutinin (PNA) polyclonal antibodies were successfully prepared. Using mouse anti-PNA polyclonal antibody as the capture antibody and rabbit anti-PNA polyclonal antibody as the detection antibody, a novel sandwich enzyme-linked immunosorbent assay (ELISA) was developed to quantify PNA. The detection limit of the ELISA method was low (0.49 ng/mL), and the linear dynamic range was between 0.78 and 100 ng/mL. The recovery ranged from 93.86% to 139.3%, whereas the intra- and inter-assay coefficients of variation were less than 10.25% and 12.06%, respectively. Sample analysis verified this method as a reliable tool for the detection of PNA in processed foods.
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