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Quantitative study of aluminum binding to human serum albumin and transferrin by a chelex competitive binding assay

1984; Elsevier BV; Volume: 125; Issue: 3 Linguagem: Inglês

10.1016/0006-291x(84)91385-8

1090-2104

Roger L. Bertholf, M. R. Wills, John Savory,

Heavy Metal Exposure and Toxicity

Binding of aluminum to human serum albumin and transferrin was investigated using a competitive binding assay incorporating a cation exchange resin, chelex. Both albumin and transferrin were found to produce linear Scatchard plots of aluminum binding data over the aluminum and protein concentration ranges found in humans. Binding constants measured for albumin and transferrin were 1.96 and 0.515 microM, respectively.