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Simultaneous Detection of High Energy Phosphates and Metabolites of Glycolysis and the Krebs Cycle by HPLC

1998; Elsevier BV; Volume: 248; Issue: 3 Linguagem: Inglês

10.1006/bbrc.1998.9005

1090-2104

Achim M. Vogt, Cordula Ackermann, Tanja Noe, Detlef Jensen, Wolfgang Kübler,

Diet and metabolism studies

For the detailed analysis of energy metabolism, a HPLC method is described allowing the single-run separation and quantification of most metabolites from glycolysis and the Krebs cycle including the high energy phosphates. With a detection limit in the picomolar range this method is even applicable when only small sample sizes of tissue are obtained.