Produção Nacional
Revisado por pares
Glutamate uptake in cultured astrocytes depends on age: a study about the effect of guanosine and the sensitivity to oxidative stress induced by H2O2
2002; Elsevier BV; Volume: 123; Issue: 10 Linguagem: Inglês
10.1016/s0047-6374(02)00069-6
ISSN1872-6216
AutoresCarmem Gottfried, Francine Tramontina, Daniela Gonçalves, Carlos Alberto Gonçalves, Emílio Hideyuki Moriguchi, Renato Dutra Dias, Susana Tchernin Wofchuk, Diogo O. Souza,
Tópico(s)Neuroinflammation and Neurodegeneration Mechanisms
ResumoRelatively few studies have been conducted to investigate the relationship between glutamate and development and/or aging. Rat cortical astrocyte cultures were used as a model to investigate glutamate uptake during development. The immunocontent of the markers glial fibrillary acidic protein (GFAP) and S100B increased, while basal secretion of S100B decreased, in astrocytes from 10 to 40 days in vitro (DIV). Basal glutamate uptake increased with age. Exposure to hydrogen peroxide decreased glutamate uptake more potently at 40 than 10 DIV. Moreover, 40 DIV astrocytes showed earlier loss of integrity (at 6 h) than 10 DIV astrocytes (at 24 h) after H2O2 exposure. Addition of guanosine stimulated glutamate uptake only in 10 DIV astrocytes. The present work shows that mature astrocytes in culture present some neurochemical alterations also observed in astrocytes of aged animals. These results can contribute to the understanding of some consequences of the excitotoxicity and oxidative stress during brain aging.
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