NF-AT-Driven Interleukin-4 Transcription Potentiated by NIP45
1996; American Association for the Advancement of Science; Volume: 274; Issue: 5294 Linguagem: Inglês
10.1126/science.274.5294.1903
ISSN1095-9203
AutoresMartin R. Hodge, Hyung J. Chun, Jyothi Rengarajan, Aya Alt, Rebecca Lieberson, Laurie H. Glimcher,
Tópico(s)Virus-based gene therapy research
ResumoThe induction of cytokine gene transcription is mediated in part by the nuclear factor of activated T cells (NF-AT). Factors involved in the mechanisms of NF-AT-mediated transcription are not well understood. A nuclear factor that interacted with the Rel homology domain (RHD) of NF-ATp was identified with the use of a two-hybrid interaction trap. Designated NIP45 (NF-AT interacting protein), it has minimal similarity to any known genes. Transcripts encoding this factor were enriched in lymphoid tissues and testes. NIP45 synergized with NF-ATp and the proto-oncogene c-Maf to activate the interleukin-4 (IL-4) cytokine promoter; transient overexpression of NIP45 with NF-ATp and c- maf in B lymphoma cells induced measurable endogenous IL-4 protein production. The identification of NIP45 advances our understanding of gene activation of cytokines, critical mediators of the immune response.
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