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Simplified method for the determination of trans monoenes in edible fats by TLC‐GLC

1992; Wiley; Volume: 69; Issue: 8 Linguagem: Inglês

10.1007/bf02635926

1558-9331

Franz Ulberth, Monika Henninger,

Advanced Chemical Sensor Technologies

Several modifications of an established thin‐layer chromatography‐gas‐liquid chromatography (TLC‐GLC) procedure for quantitating trans unsaturated fatty acids in edible fats are presented. These refinements considerably simplify the procedure without affecting accuracy. The modifications include: i) the use of pre‐coated silica sheets, dynamically impregnated with Ag + , which allow separated bands to be cut off with a pair of scissors; and ii) the use of stearic acid in the deliberately combined saturated and trans monounsaturated fatty acid methyl ester bands as an (endogenous) internal standard. Trans values thereby obtained agree favorably with the results from the conventional technique.