Isolation and characterization of microsatellite loci in the icefish Chionodraco rastrospinosus (Perciformes, Notothenioidea, Channichthyidae)

2005; Wiley; Volume: 6; Issue: 1 Linguagem: Inglês

10.1111/j.1471-8286.2005.01194.x

ISSN

1471-8286

Autores

Chiara Papetti, Lorenzo Zane, Tomaso Patarnello,

Tópico(s)

Environmental DNA in Biodiversity Studies

Resumo

Abstract We characterized eight polymorphic microsatellites in the icefish Chionodraco rastrospinosus (Perciformes, Notothenioidea, Channichthyidae). Microsatellites were isolated from a partial genomic library enriched for an AC motif. Chionodraco rastrospinosus is an endemic species inhabiting southern ocean waters surrounding the Antarctic Peninsula, the South Shetland Islands, and the South Orkney Islands. An excess of homozygotes was observed in seven out of the eight investigated loci; however, presence of null alleles was detected only for three of them suggesting that other factors may act in reducing heterozygosity. These molecular markers will be useful to investigate icefish genetic structure, possibly providing insights on its effective population size and demographic history.

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