Probing Biocatalytic Transformations with CdSe−ZnS QDs
2006; American Chemical Society; Volume: 128; Issue: 48 Linguagem: Inglês
10.1021/ja066636t
ISSN1943-2984
AutoresRon Gill, Ronit Freeman, Jian‐Ping Xu, Itamar Willner, Shira Winograd, Itzik Shweky, Uri Banin,
Tópico(s)Advanced biosensing and bioanalysis techniques
ResumoCdSe/ZnS QDs enable the optical probing of the biocatalytic oxidation of tyrosine derivatives and of the scission of peptides by thrombin. CdSe/ZnS QDs were modified with tyrosine methyl ester or with a tyrosine-containing peptide. The tyrosine units were reacted with tyrosinase/O2 to yield the respective l-DOPA and quinone derivatives. The luminescence of QDs modified by the enzyme-generated quinone units is quenched. The quinone-functionalized peptide associated with the QDs was cleaved by thrombin, a process that restored the luminescence of the QDs.
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