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Enhanced Expression of gamma/Glutamylcysteine Synthetase and Glutathione S-transferase Genes in Cisplatin-Resistant Bladder Cancer Cells with Multidrug Resistance Phenotype

1997; Lippincott Williams & Wilkins; Volume: 157; Issue: 3 Linguagem: Inglês

10.1016/s0022-5347(01)65140-1

1527-3792

Shuji Kotoh, Seiji Naito, Akira Yokomizo, Kimitoshi Kohno, Michihiko Kuwano, Joichi Kumazawa,

Sulfur Compounds in Biology

No AccessJournal of UrologyInvestigative Urology1 Mar 1997Enhanced Expression of gamma/Glutamylcysteine Synthetase and Glutathione S-transferase Genes in Cisplatin-Resistant Bladder Cancer Cells with Multidrug Resistance Phenotype Shuji Kotoh, Seiji Naito, Akira Yokomizo, Kimitoshi Kohno, Michihiko Kuwano, and Joichi Kumazawa Shuji KotohShuji Kotoh More articles by this author , Seiji NaitoSeiji Naito More articles by this author , Akira YokomizoAkira Yokomizo More articles by this author , Kimitoshi KohnoKimitoshi Kohno More articles by this author , Michihiko KuwanoMichihiko Kuwano More articles by this author , and Joichi KumazawaJoichi Kumazawa More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)65140-1AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: To elucidate the mechanisms of cisplatin resistance in human bladder cancer. Materials and Methods: After continuous exposure of KK47 cells to cisplatin, two resistant sublines, KK47/DDP10 and KK47/DDP20 were established. The glutathione content, glutathione S-transferase activity, and intracellular platinum concentration were measured while the expression of various drug resistance-related genes was examined. Results: KK47/DDP10 and KK47/DDP20 were respectively 9.3- and 18.7-fold more resistant to cisplatin than KK47, and also showed multidrug resistance. Decreased intracellular drug accumulation, increased glutathione content, elevated glutathione S-transferase activity, and an overexpression of gamma-glutamylcysteine synthetase and glutathione S-transferase pi genes were observed in the resistant sublines. Conclusions: Multiple mechanisms, including decreased drug accumulation, increased intracellular glutathione and glutathione S-transferase pi, may contribute to the acquisition of cisplatin resistance in human bladder cancer. 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Google Scholar Department of Urology and Biochemistry, Faculty of Medicine, Kyushu University, Fukuoka, Japan© 1997 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 157Issue 3March 1997Page: 1054-1058 Advertisement Copyright & Permissions© 1997 by American Urological Association, Inc.MetricsAuthor Information Shuji Kotoh More articles by this author Seiji Naito More articles by this author Akira Yokomizo More articles by this author Kimitoshi Kohno More articles by this author Michihiko Kuwano More articles by this author Joichi Kumazawa More articles by this author Expand All Advertisement PDF downloadLoading ...