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Analytical approach to the metallomic of Nile tilapia (Oreochromis niloticus) liver tissue by SRXRF and FAAS after 2D-PAGE separation: Preliminary results

2010; Elsevier BV; Volume: 82; Issue: 3 Linguagem: Inglês

10.1016/j.talanta.2010.06.023

1873-3573

Paula Monteiro de Lima, Renato de Cássio Ferreira Neves, Felipe André dos Santos, Carlos A. Pérez, Marcelo O.A. da Silva, Marco Aurélio Zezzi Arruda, Gustavo R. Castro, Pedro de Magalhães Padilha,

Heavy Metal Exposure and Toxicity

An investigation was made into calcium, iron and zinc in protein spots in samples of Nile tilapia (Oreochromis niloticus) liver tissue obtained after protein separation by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and subsequent qualitative and quantitative evaluation by synchrotron radiation X-ray fluorescence (SRXRF) and Flame Atomic Absorption Spectrometry (FAAS). An analysis of the fluorescence spectra indicated the presence of calcium, iron and zinc in 12, 6 and 8 liver protein spots, respectively. The metal ions found were distributed mainly in proteins with a molar mass of less than 40.00 kDa and more than 12.00 kDa, with pI in the range of 4.70–9.40. The only exception was a spot presenting protein with a molar mass of 10.10 kDa. In addition to calcium, iron and zinc, sulfur and phosphorus – which are non-metals that may be part of the protein structure, were also detected. After microwave-assisted acid mineralization of the proteins spots, a FAAS estimation of the concentration of calcium, iron and zinc ions bound to these proteins indicated a range of 1.08–5.80 mg g−1, 2.02–8.03 mg g−1 and 1.60–8.55 mg g−1, respectively.