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Assessment of serological tests for the diagnosis of canine visceral leishmaniasis

2012; Elsevier BV; Volume: 195; Issue: 2 Linguagem: Inglês

10.1016/j.tvjl.2012.06.010

1532-2971

Denise Amaro da Silva, Maria de Fátima Madeira, Tuanne Rotti Abrantes, Carlos José de Lima Barbosa Filho, Fabiano Borges Figueiredo,

Virus-based gene therapy research

An immunoenzymatic assay (ELISA), an indirect immunofluorescence antibody test (IFAT) with different antigens (ELISA-Leishmania chagasi, ELISA-L. major-like, IFAT-L. chagasi and IFAT-L. major-like), and an immunochromatographic test were assessed for the diagnosis of canine visceral leishmaniasis (CVL). Serum samples from 144 dogs from an endemic area for visceral leishmaniasis in the municipality of Rio de Janeiro were tested. The sensitivities of the serological tests were 93%, 100%, 73%, 60% and 93%, with specificities of 87%, 92%, 77%, 96% and 92% for the ELISA-L. major-like, ELISA-L. chagasi, IFAT-L. major-like, IFAT-L. chagasi and the immuno chromatographic test, respectively. ELISA-L. chagasi was the best test for the diagnosis of CVL, but the immunochromatographic test could be a useful alternative as it offers simple and rapid diagnosis without the need for a specialized laboratory.