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RECOVERY OF FISH BONE FROM HOKI (JOHNIUS BELENGERI) FRAME USING A PROTEOLYTIC ENZYME ISOLATED FROM MACKEREL INTESTINE

2003; Wiley; Volume: 27; Issue: 3 Linguagem: Inglês

10.1111/j.1745-4514.2003.tb00280.x

1745-4514

Se‐Kwon Kim, Pyo‐Jam Park, Hee‐Guk Byun, Jae‐Young Je, Sung-Hoon Moon, Soohyun Kim,

Animal Genetics and Reproduction

Journal of Food BiochemistryVolume 27, Issue 3 p. 255-266 RECOVERY OF FISH BONE FROM HOKI (JOHNIUS BELENGERI) FRAME USING A PROTEOLYTIC ENZYME ISOLATED FROM MACKEREL INTESTINE SE-KWON KIM, Corresponding Author SE-KWON KIM Department of Chemistry Pukyong National University Pusan 608–737, South Korea To whom correspondence should be addressed. Pukyong National University, 599–1, Daeyon 3-dong, Nam-Gu, Pusan 608–737, Korea. TEL: +82–51–620–6375; FAX: +82–51–628–8147, EMAIL: [email protected]Search for more papers by this authorPYO-JAM PARK, PYO-JAM PARK Department of Chemistry Pukyong National University Pusan 608–737, South KoreaSearch for more papers by this authorHEE-GUK BYUN, HEE-GUK BYUN Department of Chemistry Pukyong National University Pusan 608–737, South KoreaSearch for more papers by this authorJAE-YOUNG JE, JAE-YOUNG JE Department of Chemistry Pukyong National University Pusan 608–737, South KoreaSearch for more papers by this authorSUNG-HOON MOON, SUNG-HOON MOON Department of Chemistry Pukyong National University Pusan 608–737, South KoreaSearch for more papers by this authorSOO-HYUN KIM, SOO-HYUN KIM Department of Food Science and Engineering Cheju National University Cheju 690–756, KoreaSearch for more papers by this author SE-KWON KIM, Corresponding Author SE-KWON KIM Department of Chemistry Pukyong National University Pusan 608–737, South Korea To whom correspondence should be addressed. Pukyong National University, 599–1, Daeyon 3-dong, Nam-Gu, Pusan 608–737, Korea. TEL: +82–51–620–6375; FAX: +82–51–628–8147, EMAIL: [email protected]Search for more papers by this authorPYO-JAM PARK, PYO-JAM PARK Department of Chemistry Pukyong National University Pusan 608–737, South KoreaSearch for more papers by this authorHEE-GUK BYUN, HEE-GUK BYUN Department of Chemistry Pukyong National University Pusan 608–737, South KoreaSearch for more papers by this authorJAE-YOUNG JE, JAE-YOUNG JE Department of Chemistry Pukyong National University Pusan 608–737, South KoreaSearch for more papers by this authorSUNG-HOON MOON, SUNG-HOON MOON Department of Chemistry Pukyong National University Pusan 608–737, South KoreaSearch for more papers by this authorSOO-HYUN KIM, SOO-HYUN KIM Department of Food Science and Engineering Cheju National University Cheju 690–756, KoreaSearch for more papers by this author First published: 23 February 2007 https://doi.org/10.1111/j.1745-4514.2003.tb00280.xCitations: 20AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL ABSTRACT A new enzymatic treatment procedure was devised to recover and further utilize fish bones from processing which are normally discarded. This procedure includes the use of an enzyme preparation isolated from raw mackerel intestine by acetone precipitation. The caseinolytic activity of mackerel intestine crude enzyme (MICE) was approximately 0.58 U/mg protein, which was comparable to those of trypsin and α-chymotrypsin, or even higher than those of papain and Aspergillus saitoi protease. The optimum pH, temperature and enzyme/substrate ratio of MICE for hydrolysis of protein with hoki (Johnius belengeri) frames were determined to be pH 9.0, 40C and 1:100 (w/w). When the hoki frame was treated by MICE for 6 h under the optimum reaction conditions, the yield of bone recovery was approximately 90%. Compared to Alcalase, trypsin, α-chymotrypsin and Neutrase, the yield of bone recovery using MICE was higher. 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