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A new experimental model of cigarette smoke-induced emphysema in Wistar rats

2014; Sociedade Brasileira de Pneumologia e Tisiologia; Volume: 40; Issue: 1 Linguagem: Inglês

10.1590/s1806-37132014000100007

1806-3756

Rodrigo de las Heras Kozma, Edson Marcelino Alves, Valter Abraão Barbosa-de-Oliveira, Fernanda Degobbi Tenório Quirino dos Santos Lopes, Renan Cenize Guardia, Henrique Vivi Buzo, Carolina Arruda de Faria, Camila Yamashita, Manzélio Cavazzana, Fernando Frei, Maria José de Oliveira Ribeiro-Paes, João Tadeu Ribeiro‐Paes,

Neuroscience of respiration and sleep

OBJECTIVE: To describe a new murine model of cigarette smoke-induced emphysema. METHODS: Twenty-four male Wistar rats were divided into two groups: the cigarette smoke group, comprising 12 rats exposed to smoke from 12 commercial filter cigarettes three times a day (a total of 36 cigarettes per day) every day for 30 weeks; and the control group, comprising 12 rats exposed to room air three times a day every day for 30 weeks. Lung function was assessed by mechanical ventilation, and emphysema was morphometrically assessed by measurement of the mean linear intercept (Lm). RESULTS: The mean weight gain was significantly (approximately ten times) lower in the cigarette smoke group than in the control group. The Lm was 25.0% higher in the cigarette smoke group. There was a trend toward worsening of lung function parameters in the cigarette smoke group. CONCLUSIONS: The new murine model of cigarette smoke-induced emphysema and the methodology employed in the present study are effective and reproducible, representing a promising and economically viable option for use in studies investigating the pathophysiology of and therapeutic approaches to COPD.