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Protease‐activated receptor‐2 downregulation is associated to vitiligo lesions

2009; Wiley; Volume: 22; Issue: 3 Linguagem: Inglês

10.1111/j.1755-148x.2009.00562.x

1755-148X

Silvia Moretti, Romina Nassini, Francesca Prignano, Alessandra Pacini, Serena Materazzi, Antonella Naldini, Antonella Simoni, Gianna Baroni, Silvia Pellerito, Irene Filippi, Torello Lotti, Pierangelo Geppetti, Daniela Massi,

melanin and skin pigmentation

Appendix S1. Protease-activated receptor-2 downregulation is associated to vitiligo lesions. Figure S1. Immunohistochemical expression of PAR-2 and tyrosinase in non-vitiligo leukodermas: A, upper row) Biopsy from an achromic patch of pytiriasis versicolor shows a mild perivascular dermatitis (haematoxylin & eosin stain); at higher power numerous hyphae are present within a compact horny layer (PAS-D stain, inset); scattered epidermal melanocytes appear positive for TYR; PAR-2 is diffusely and strongly expressed within the epidermis. B, lower row). Biopsy from an achromic patch of lichen simplex chronicus shows compact hyperorthokeratosis, irregular epidermal hyperplasia and mild fibroplasia in the superficial dermis (haematoxylin & eosin stain); TYR positive melanocytes are evident at the basal cell layer; PAR-2 is diffusely and moderately expressed within the epidermis. (Scale bar = 50 μm) (streptavidin–biotin peroxidase complex/AEC). Figure S2. Typical tracings and pooled data of mobilization of intracellular Ca2+ ([Ca2+]i) in primary cultures of non-lesional (grey lines or columns) or lesional (black lines or columns) keratinocytes obtained from patients with vitiligo by increasing concentrations of the PAR-2 activating (AP, SLIGKV-NH2) or reverse (RP, VKGILS-NH2) peptides (A, B) and the PAR-1 activating (AP, TFLLRN-NH2) or reverse (RP, NRLLFT-NH2) peptides (C, D). Each column is the mean ± SEM of at least 15 observations. *P < 0.05 versus non-lesional primary keratinocytes. Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.