Repeated use of a hydrophobic ligand-containing porous membrane for protein recovery
1997; Elsevier BV; Volume: 134; Issue: 1 Linguagem: Inglês
10.1016/s0376-7388(97)00143-9
ISSN1873-3123
AutoresNoboru KUBOTA, Minoru Kounosu, Kyoichi Saito, Kazuyuki Sugita, Kohei Watanabe, Takanobu Sugo,
Tópico(s)Advanced Drug Delivery Systems
ResumoA porous hollow-fiber membrane containing a phenyl group as a hydrophobic ligand was prepared by radiation-induced graft polymerization of glycidyl methacrylate, followed by successive ring-opening reactions with phenol and water. Bovine serum albumin (BSA) was bound to the ligand during permeation of a BSA solution in phosphate buffer containing 2M (NH4)2SO4 through the pores of the hollow fiber. Subsequent elution with an (NH4)2SO4-free buffer exhibited an elution percentage of 82%. Repeated cycles of adsorption and elution caused the accumulation of BSA on the pore surface, resulting in a decrease in the binding capacity of BSA with increasing number of cycles. In contrast, by permeating 1 M NaOH after each elution, the binding capacity of BSA was maintained even after ten cycles. This alkaline regeneration was found to be effective in ensuring repeated use of the phenyl-group-containing porous membrane for recovery of proteins.
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