Determination of dimethylarginine dimethylaminohydrolase activity in the kidney
2007; Elsevier BV; Volume: 72; Issue: 7 Linguagem: Inglês
10.1038/sj.ki.5002446
ISSN1523-1755
Autores Tópico(s)Adenosine and Purinergic Signaling
ResumoDimethylarginine dimethylaminohydrolase (DDAH) metabolizes asymmetric dimethylarginine to generate L-citrulline and is present in large quantities in the kidney. We present a new study that optimizes the Prescott–Jones colorimetric assay to measure DDAH-dependent L-citrulline generation in kidney homogenates. We found that the removal of urea with urease is necessary since urea also produces a positive reaction. Deproteinization with sulfosalicylic acid was found to be optimal and that protease inhibitors were not necessary. All assays were conducted in phosphate buffer, since other common additives can create false positive and false negative reactions. Arginase or nitric oxide synthase isoenzymes were not found to influence L-citrulline production. Our optimized L-citrulline production assay to measure DDAH activity correlated closely with the direct measure of the rate of asymmetric dimethylarginine consumption. Using this assay, we found that both superoxide and nitric oxide inhibit renal cortical DDAH activity in vitro. Dimethylarginine dimethylaminohydrolase (DDAH) metabolizes asymmetric dimethylarginine to generate L-citrulline and is present in large quantities in the kidney. We present a new study that optimizes the Prescott–Jones colorimetric assay to measure DDAH-dependent L-citrulline generation in kidney homogenates. We found that the removal of urea with urease is necessary since urea also produces a positive reaction. Deproteinization with sulfosalicylic acid was found to be optimal and that protease inhibitors were not necessary. All assays were conducted in phosphate buffer, since other common additives can create false positive and false negative reactions. Arginase or nitric oxide synthase isoenzymes were not found to influence L-citrulline production. Our optimized L-citrulline production assay to measure DDAH activity correlated closely with the direct measure of the rate of asymmetric dimethylarginine consumption. Using this assay, we found that both superoxide and nitric oxide inhibit renal cortical DDAH activity in vitro. Dimethylarginine dimethylaminohydrolase (DDAH) metabolizes the methylarginines asymmetric dimethylarginine (ADMA) and NW-monomethyl-L-arginine, to generate L-citrulline, and is highly expressed in the kidney.1.Leiper J.M. Santa Maria J. Chubb A. et al.Identification of two human dimethylarginine dimethylaminohydrolases with distinct tissue distributions and homology with microbial arginine diminishes.Biochem J. 1999; 343: 209-214Crossref PubMed Scopus (434) Google Scholar ADMA is elevated in many systemic diseases, including renal failure, possibly due to impaired renal DDAH activity. DDAH activity can be measured by rate of substrate (e.g. ADMA) consumption, but these assays are time consuming and costly.2.MacAllister R.J. Parry H. Kimoto M. et al.Regulation of nitric oxide synthesis by dimethylarginine dimethylaminohydrolase.Br J Pharmacol. 1996; 119: 1533-1540Crossref PubMed Scopus (368) Google Scholar,3.Ito A. Tsao P.S. Adimoolam S. et al.Novel mechanism for endothelial dysfunction: dysregulation of dimethylarginine dimethylaminohydrolase.Circulation. 1999; 99: 3092-3095Crossref PubMed Scopus (612) Google Scholar A colorimetric method that detects L-citrulline production can also be used providing that (1) other pathways that generate or remove L-citrulline are inactivated and (2) interfering compounds have been removed. Here, we have optimized the Prescott–Jones method4.Prescott L.M. Jones M.E. Modified methods for the determination of carbamyl aspartate.Anal Biochem. 1969; 32: 408-419Crossref PubMed Scopus (333) Google Scholar using diacetyl monoxime derivatization of the ureido group in L-citrulline to form color5.Boyde T.R. Rahmatullah M. Optimization of conditions for the colorimetric determination of L-citrulline, using diacetyl monoxime.Anal Biochem. 1980; 107: 424-431Crossref PubMed Scopus (271) Google Scholar,4.Prescott L.M. Jones M.E. Modified methods for the determination of carbamyl aspartate.Anal Biochem. 1969; 32: 408-419Crossref PubMed Scopus (333) Google Scholar that has been adapted to a 96-well format.6.Knipp M. Vasak M. A colorimetric 96-well microtiter plate assay for the determination of enzymatically formed L-citrulline.Anal Biochem. 2000; 286: 257-264Crossref PubMed Scopus (165) Google Scholar Particular attention was paid to nonspecific color generation by urea.7.Gornall A.G. Hunter A. A colorimetric method for the determination of L-citrulline.Biochem J. 1941; 35: 650-658Crossref PubMed Google Scholar Furthermore, we compared this modified L-citrulline assay to the direct high-pressure liquid chromatography method measuring rate of ADMA consumption. The enzyme is saturated at between 100 μM and 1 mM substrate (ADMA) and we therefore use 1 mM ADMA in all studies. We compared four different homogenization buffers, and found sodium phosphate buffer, pH=6.5, was without effect on color formation (Table 1). Without deproteinization, both bovine serum albumin and the kidney homogenate caused turbidity. Sulfosalicylic acid (4%) gave the lowest blank absorbance and was used for deproteinization. With deproteinization, there was no background color with bovine serum albumin although the kidney homogenate still had high color, suggesting the presence of interfering factors.Table 1Effect of buffers and additives on the L-citrulline assay in the presence of 25 μM L-citrullineL-citrulline μMColor as % of controlHomogenization bufferaHB1, pH=6.8 contained 20 mM Tris, 1% Triton X-100, 5 mM EDTA, 10 mM EGTA, 2 mM DTT, 1 mM sodium orthovanadate, 0.1 mg/ml phenylmethylsulfonyl fluoride, 10 μg/ml leupeptin, and aprotinin; HB2 contained 0.1 M sodium phosphate, pH=6.5 containing 2 mM 2-mercaptoethanol;10 HB3 contained 0.1 M sodium phosphate, pH=6.5;12 and HB4 was RIPA buffer (Santa Cruz, CA, USA), containing 20 mM Tris, pH=7.6, 137 mM sodium chloride, 0.2% Nonidet P-40, 0.1% sodium deoxycholate, 0.02% SDS, 0.0008% sodium azide, and protease inhibitors. HB117.0±0.868 HB2<Minimal 100bThe supernatant was opalescent.464Buffer base 1% Triton13.0±0.952 1 M HEPES27.5±0.4110 0.3 M sucrose>100500 0.9% normal saline24.6±0.698 0.1 M sodium phosphate24.9±0.6100 100 mM urea>1001419Additives 0.1 M DTT>100384 1% 2-mercaptoethanol<Minimal 100bThe supernatant was opalescent.415Protein with deproteinization 1 mg/ml BSA26.1±0.8107 2 mg/ml BSA25.7±0.8103 1 mg/ml kidney homogenate63.4±1.1253 2 mg/ml kidney homogenate82.6±1.2330ADMA, asymmetric dimethylarginine; BSA, bovine serum albumin; EDTA, ethylenediaminetetraacetic acid; EGTA, ethylene glycol-bis(â-aminoethyl ether)-N,N,N',N',-tetraacetic acid; HEPES, hydroxyethylpiperazine-N′-2-ethanesulfonic; SDS, sodium dodecyl sulfate; DTT, DL-dithiothreitol.The values reflect effect of an additive on the color generated by a 25 μML-citrulline standard (taken as 100%).a HB1, pH=6.8 contained 20 mM Tris, 1% Triton X-100, 5 mM EDTA, 10 mM EGTA, 2 mM DTT, 1 mM sodium orthovanadate, 0.1 mg/ml phenylmethylsulfonyl fluoride, 10 μg/ml leupeptin, and aprotinin; HB2 contained 0.1 M sodium phosphate, pH=6.5 containing 2 mM 2-mercaptoethanol;10.Ogawa T. Kimoto M. Sasaoka K. Purification and properties of a new enzyme NG NG-dimethylarginine dimethylaminohydrolase, from rat kidney.J Biol Chem. 1989; 264: 10205-10209Abstract Full Text PDF PubMed Google Scholar HB3 contained 0.1 M sodium phosphate, pH=6.5;12.Yin Q.F. Xiong Y. Pravastatin restores DDAH activity and endothelium-dependent relaxation of rat aorta after exposure to glycated protein.J Cardiovasc Pharmacol. 2005; 45: 525-532Crossref PubMed Scopus (50) Google Scholar and HB4 was RIPA buffer (Santa Cruz, CA, USA), containing 20 mM Tris, pH=7.6, 137 mM sodium chloride, 0.2% Nonidet P-40, 0.1% sodium deoxycholate, 0.02% SDS, 0.0008% sodium azide, and protease inhibitors.b The supernatant was opalescent.c Protease inhibitors: 0.1 mg/ml phenylmethylsulfonyl fluoride, 10 μg/ml leupeptin, and aprotinin. Open table in a new tab ADMA, asymmetric dimethylarginine; BSA, bovine serum albumin; EDTA, ethylenediaminetetraacetic acid; EGTA, ethylene glycol-bis(â-aminoethyl ether)-N,N,N',N',-tetraacetic acid; HEPES, hydroxyethylpiperazine-N′-2-ethanesulfonic; SDS, sodium dodecyl sulfate; DTT, DL-dithiothreitol. The values reflect effect of an additive on the color generated by a 25 μML-citrulline standard (taken as 100%). As shown in Figure 1, the high background color seen in the deproteinized kidney homogenate was reduced by >95% at t=15 min after incubation with urease. Addition of 1, 5, 10, 50, and 100 mM urea gave color equivalent to ∼21, 49, 106, 195, and 221 μM L-citrulline, respectively, but gave no background color at t=0 when preincubated with urease for 15 min. After preincubation with urease, citrulline production in kidney and other tissue homogenates incubated with 1 mM ADMA was linear from 0 to 120 min (Figure 2). We used a 45-min incubation in subsequent studies. In kidney cortex, the rate of production of L-citrulline=0.3976 μM/g protein/min and the corresponding rate of consumption of ADMA=0.4378 μM/g protein/min are similar (Figure 3), suggesting that this assay gives a faithful measurement of DDAH activity. Other tests (Figure S2) confirmed that there was no citrulline to arginine conversion and no citrulline generation by activity of arginase or nitric oxide synthase. The renal cortex and medulla DDAH activity was 0.39±0.01 (n=15) and 0.30±0.01 (n=3) μM/g protein/min, respectively. Inter- and intra-assay coefficient of variation are 5.61±0.28% (n=12) and 4.82±0.19% (n=9). The NO donors and superoxide donor inhibited DDAH activity (Figure 4). We have used the Prescott–Jones method to measure kidney DDAH activity from rate of L–citrulline production and found that urea markedly raises background and must be removed; deproteinization is essential and the choice of deproteinization method influences background color; the modified method correlates well with rate of ADMA consumption; and both superoxide and NO, known to inhibit DDAH activity, produce declines in rate of L-citrulline formation in kidney homogenates. Knipp and Vasak6.Knipp M. Vasak M. A colorimetric 96-well microtiter plate assay for the determination of enzymatically formed L-citrulline.Anal Biochem. 2000; 286: 257-264Crossref PubMed Scopus (165) Google Scholar adapted the Prescott–Jones assay to a 96-well plate method for measurement of activity of the purified DDAH enzyme. However, in complex tissues, there may be agents that reduce or increase color development and alter L-citrulline metabolism. Of particular note, the development of color is not specific for L-citrulline but also occurs with urea,7.Gornall A.G. Hunter A. A colorimetric method for the determination of L-citrulline.Biochem J. 1941; 35: 650-658Crossref PubMed Google Scholar and there is a urea concentration gradient in kidney (∼4 mM in cortex and ∼20 mM in inner medulla).8.Safirstein R. Miller P. Kahn T. Cortical and papillary absorptive defects in gentamicin nephrotoxicity.Kidney Int. 1983; 24: 526-533Abstract Full Text PDF PubMed Scopus (10) Google Scholar Even in renal cortex, urea accounts for more than 90% of baseline absorbance, and therefore obscures DDAH-induced changes in color due to citrulline formation. In the presence of urease, the effect of urea (up to 100 mM) can be completely removed from kidney cortex and medulla. Kulhanek et al.9.Kulhanek V. Maderova V. Sindelarova K. Vojtiskova V. Modified determination of ornithine-carbamyl-transferase activity in biological material.Clin Chim Acta. 1963; 8: 579-585Crossref PubMed Scopus (3) Google Scholar reported that urease treatment was not required for citrulline assay in liver and brain. Our results, however, demonstrate that where urea concentration is measurable, urease should be used. Although the urea effect can be prevented by initial ion-exchange chromatography,10.Ogawa T. Kimoto M. Sasaoka K. Purification and properties of a new enzyme NG NG-dimethylarginine dimethylaminohydrolase, from rat kidney.J Biol Chem. 1989; 264: 10205-10209Abstract Full Text PDF PubMed Google Scholar this is more costly and time consuming compared to urease. Another difficulty is that the diacetyl monoxime reagent can detect protein-bound L-citrulline as well as free L-citrulline. Although no separate protein-removing step was required in the purified enzyme system,6.Knipp M. Vasak M. A colorimetric 96-well microtiter plate assay for the determination of enzymatically formed L-citrulline.Anal Biochem. 2000; 286: 257-264Crossref PubMed Scopus (165) Google Scholar in tissues, protein precipitation is mandatory. We found that 4% sulfosalicylic acid gave lowest background. Buffer/additives also influence color development, for example, 2-mercaptoethanol (in HB2) reduces color formation,5.Boyde T.R. Rahmatullah M. Optimization of conditions for the colorimetric determination of L-citrulline, using diacetyl monoxime.Anal Biochem. 1980; 107: 424-431Crossref PubMed Scopus (271) Google Scholar which might explain our observation of a higher renal DDAH activity than a previous study using HB2.10.Ogawa T. Kimoto M. Sasaoka K. Purification and properties of a new enzyme NG NG-dimethylarginine dimethylaminohydrolase, from rat kidney.J Biol Chem. 1989; 264: 10205-10209Abstract Full Text PDF PubMed Google Scholar Without urease treatment, the high background color due to urea obscures DDAH-dependent L-citrulline formation until ∼t=45 min (Figure S1), which might also explain the longer incubation time used previously.11.Stuhlinger M.C. Tsao P.S. Her J.H. et al.Homocysteine impairs the nitric oxide synthase pathway: role of asymmetric dimethylarginine.Circulation. 2001; 104: 2569-2575Crossref PubMed Scopus (603) Google Scholar, 12.Yin Q.F. Xiong Y. Pravastatin restores DDAH activity and endothelium-dependent relaxation of rat aorta after exposure to glycated protein.J Cardiovasc Pharmacol. 2005; 45: 525-532Crossref PubMed Scopus (50) Google Scholar, 13.Chen Y. Li Y. Zhang P. et al.Dimethylarginine dimethylaminohydrolase and endothelial dysfunction in failing hearts.Am J Physiol Heart Circ Physiol. 2005; 289: H2212-H2219Crossref PubMed Scopus (60) Google Scholar In addition to DDAH, ornithine carbamoyltransferase and nitric oxide synthase generate L-citrulline. In this assay, activity of both enzymes can be ignored since ornithine carbamoyltransferase is not detectable in kidney14.Edmonds M.S. Lowry K.R. Baker D.H. Urea cycle metabolism: effects of supplemental ornithine or L-citrulline on performance, tissue amino acid concentrations and enzymatic activity in young pigs fed arginine-deficient diets.J Anim Sci. 1987; 65: 706-716PubMed Google Scholar and 1 mM ADMA used as substrate is a potent nitric oxide synthase inhibitor. On the other hand, L-citrulline can be converted to L-arginine by argininosuccinate synthase and lyase. However, in tissue homogenate, citrulline consumption by argininosuccinate synthase and lyase requires added aspartate15.Ratner S. Petrack B. Biosynthesis of urea. IV. Further studies on condensation in arginine synthesis from citrulline.J Biol Chem. 1953; 200: 161-174Abstract Full Text PDF PubMed Google Scholar and ATP, and we found no citrulline consumption under the conditions of our assay (Supplementary Material). Furthermore, arginases, which might indirectly increase L-citrulline consumption by increasing rate of L-arginine utilization,16.Curis E. Nicolis I. Moinard C. et al.Almost all about L-citrulline in mammals.Amino Acids. 2005; 29: 177-205Crossref PubMed Scopus (397) Google Scholar are not active since arginase inhibition did not affect L-citrulline formation and there was no l-arginine consumption. Download .doc (.06 MB) Help with doc files Supplementary Data Both oxidative and nitrosative stress have been reported to inhibit DDAH activity,17.Knipp M. How to control NO production in cells: N(omega), N(omega)-dimethyl-L-arginine dimethylaminohydrolase as a novel drug target.Chembiochem. 2006; 7: 879-889Crossref PubMed Scopus (28) Google Scholar,18.Leiper J. Murray-Rust J. McDonald N. Vallance P. S-nitrosylation of dimethylarginine dimethylaminohydrolase regulates enzyme activity: further interactions between nitric oxide synthase and dimethylarginine dimethylaminohydrolase.Proc Natl Acad Sci USA. 2002; 99: 13527-13532Crossref PubMed Scopus (273) Google Scholar and in this study we show that both superoxide and NO donors have an acute inhibitory action on renal cortex DDAH activity, measured from L-citrulline production. In conclusion, this colorimetric assay of L-citrulline accumulation is a simple and inexpensive method optimized for detection of renal tissue DDAH activity in vitro, which agrees well with the more costly and time-consuming method of measuring ADMA consumption. This can also be adapted for other tissues, even with low activity such as cerebellum, but should be optimized before use. Male Sprague–Dawley rats from Harlan (Indianapolis, IN, USA) were used. Tissues were collected after perfusion with cold phosphate-buffered saline and stored at -80°C. Protein concentration was determined by Bradford assay. Tissue homogenate was adjusted to the concentration of 20 mg/ml. Several pilot studies were conducted to optimize the assay including evaluation of homogenization buffer, deproteinization reagents, and other pathways of citrulline metabolism (Supplementary Material & Table S1). Recommended assay procedures are summarized in Table 2. A time-course study was conducted with preincubation of urease with homogenates of rat kidney cortex, liver, cerebellum, and aorta, then incubation with 1 mM ADMA from 0 to 120 min. We also investigated the impact of NO and superoxide (diethylamine NONOate, sodium nitrate, and 2,3-dimethoxy-1,4-naphthoquinone) on DDAH activity.Table 2Recommend assay procedures/conditions for the measurement of renal cortical DDAH activity1.Homogenize tissue with 5 × sodium phosphate buffer, pH=6.52.Adjust protein concentration to 20 mg/ml3.Preincubate urease (100 U/ml homogenate) with tissue homogenate in 37°C water bath for 15 min4.Add 100 μl sample to 400 μl 1 mM ADMA in sodium phosphate buffer (respective blank is sample omitting ADMA)5.Incubate mixture in 37°C water bath for 45 min6.Stop reaction by addition of 0.5 ml of 4% sulfosalicylic acid7.Vortex and centrifuge at 3000 g for 10 min8.Add 100 μl supernatant into a 96-well plate in triplicate9.Serially dilute 100 μm L-citrulline standard to 0, 3.125, 6.25, 12.5, 25, 50, and 100 μm10.Add 100 μl of standard into the 96-well plate in triplicate11.Mix one part of oxime reagent with two parts of antipyrine/H2SO4 reagent to make the 'color mixture'12.Add 100 μl of color mixture into the wells13.Cover the plate with a sealing tape14.Shake on a plate shaker for 1 min15.Incubate the plate in 60°C water bath for 110 min in the dark16.Cool the plate in an ice bath for 10 min17.Measure the absorbance by spectrophotometric analysis at 466 nm18.Subtract the value of respective blank19.The DDAH activity is represented as μm L-citrulline formation/g protein/min at 37°CADMA, asymmetric dimethylarginine; DDAH, dimethylarginine dimethylaminohydrolase. Open table in a new tab ADMA, asymmetric dimethylarginine; DDAH, dimethylarginine dimethylaminohydrolase. We compared the rate of L-citrulline production by DDAH with the rate of ADMA degradation at t=0, 30, 45, 90, and 120 min. In this study, 400 μl of 1 mM ADMA was mixed with the 100 μl of kidney homogenate (20 mg/ml), and 100 μl of the mixture was collected for high-pressure liquid chromatography analysis of ADMA at the various times, as shown above. ADMA (and L-arginine) levels were measured in tissue homogenate using reverse-phase high-pressure liquid chromatography with the Waters AccQ-Fluor fluorescent reagent kit as published previously.19.Tain Y.L. Freshour G. Dikalova A. et al.Vitamin E reduces glomerulosclerosis, restores renal neuronal NOS, and suppresses oxidative stress in the 5/6 nephrectomized rat.Am J Physiol Renal Physiol. 2007; 292: F1404-F1410Crossref PubMed Scopus (77) Google Scholar Data are presented as mean±s.e.m. The effects of arginase inhibitor, NO, and superoxide were compared by unpaired t-test. The correlation between L-citrulline formation and ADMA consumption was analyzed by Pearson's correlation coefficient. These studies were supported by NIH Grant R01DK056843 and a BRP from Florida Department of Health. We thank Dr Sidney M Morris, Jr for discussion. The technical assistance of Harold Snellen is gratefully acknowledged. Supplementary Methods and Results. Optimization of homogenization buffers. Table S1. Effect of deproteinization reagents on absorbance of blank. Figure S1. Time course of color formation in citrulline equivalents in the presence of the DDAH substrate (ADMA), and in the absence (solid circle) and presence of urease (open circle). Figure S2. The effect of arginase on the l -citrulline assay to detect renal DDAH activity.
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