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Role of TLR-2 and Fungal Surface Antigens on Innate Immune Response Against Sporothrix schenckii

2012; Taylor & Francis; Volume: 42; Issue: 1 Linguagem: Inglês

10.3109/08820139.2012.719982

1532-4311

Thaís de Cássia Negrini, Lucas Souza Ferreira, Pâmela Alegranci, Rodrigo Alex Arthur, Pedro Passos Sundfeld, Danielle Cardoso Geraldo Maia, Luís Carlos Spolidório, Iracilda Zeppone Carlos,

Plant Pathogens and Fungal Diseases

Sporotrichosis is an infection caused by the dimorphic fungus Sporothrix schenckii. Toll-like receptors (TLRs) play an important role in immunity, since they bind to pathogen surface antigens and initiate the immune response. However, little is known about the role of TLR-2 and fungal surface antigens in the recognition of S. schenckii and in the subsequent immune response. This study aimed to evaluate the involvement of TLR-2 and fungal surface soluble (SolAg) and lipidic (LipAg) antigens in phagocytosis of S. schenckii and production of immune mediators by macrophages obtained from WT and TLR-2-/- animals. The results showed that TLR-2-/- animals had had statistical lower percentage of macrophages with internalized yeasts compared to WT. SolAg and LipAg impaired phagocytosis and immunological mediator production for both WT and TLR-2-/-. The absence of TLR-2 led to lower production of the cytokines TNF-α, IL-1β, IL-12 and IL-10 compared to WT animals. These results suggest a new insight in relation to how the immune system, through TLR-2, recognizes and induces the production of mediators in response to the fungus S. schenckii.